Nanoelectronic Investigation Reveals the Electrochemical Basis of Electrical Conductivity in Shewanella and Geobacter
Nanoelectronic Investigation Reveals the Electrochemical Basis of Electrical Conductivity in Shewanella and Geobacter
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DOI：
10.1021/acsnano.6b03655
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发表时间：
2016-11-01
期刊：
影响因子：
17.1
通讯作者：
Duan, Xiangfeng
中科院分区：
文献类型：
--
作者：
Ding, Mengning;Shiu, Hui-Ying;Duan, Xiangfeng
在希瓦氏菌和Geophylla spp中测量的电导率。是一个有趣的物理性质，是可能的细胞外电子传递（EET）途径的基本基础。关于这些微生物细胞结构中报道的电导率的起源存在相当大的争论，这对于破译EET机制至关重要。在这里，我们报告了希瓦氏菌和Geophylla spp的系统的芯片上纳米电子调查。在生理条件下，以阐明单个微生物细胞和生物膜的电导率的复杂基础。同时在少数细胞和生物膜水平下对活希瓦氏菌进行的电学和电化学测量表明，表观电导率可以追溯到细胞/电极界面处基于电化学的电子转移。我们进一步表明，类似的结果和结论适用于Geophysicspp。总之，我们的研究提供了重要的见解，以前提出的物理模型，关于微生物的电导率，以及EET途径希瓦氏菌和地芽孢杆菌属。
The electrical conductivity measured in Shewanella and Geobacter spp. is an intriguing physical property that is the fundamental basis for possible extracellular electron transport (EET) pathways. There is considerable debate regarding the origins of the electrical conductivity reported in these microbial cellular structures, which is essential for deciphering the EET mechanism. Here, we report systematic on-chip nanoelectronic investigations of both Shewanella and Geobacter spp. under physiological conditions to elucidate the complex basis of electrical conductivity of both individual microbial cells and biofilms. Concurrent electrical and electrochemical measurements of living Shewanella at both few cell and the biofilm levels indicate that the apparent electrical conductivity can be traced to electrochemical -based electron transfer at the cell/electrode interface. We further show that similar results and conclusions apply to the Geobacter spp. Taken together, our study offers important insights into previously proposed physical models regarding microbial conductivities as well as EET pathways for Shewanella and Geobacter spp.