The Bactericidal Effect of MA-T for Factitiously Contaminated and Used Masks
The Bactericidal Effect of MA-T for Factitiously Contaminated and Used Masks
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DOI：
10.1248/bpb.b22-00046
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发表时间：
2022-06-01
影响因子：
2
通讯作者：
Sakai, Takayoshi
中科院分区：
文献类型：
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作者：
Urakawa, Ryuta;Shibata, Takekatsu;Sakai, Takayoshi
匹配转换系统（MA-T）是一种按需二氧化氯水溶液，是一种出色的安全消毒剂，因为二氧化氯在储存期间或使用前不会被检测到。MA-T中二氧化氯的产生是在目标微生物存在下通过催化反应诱导的。在这项研究中，我们调查了MA-T消毒口罩作为一种重复使用的方法，以消除口罩短缺。将大肠杆菌喷洒在消毒后的外科口罩上，对人工污染的口罩进行MA-T喷洒或浸泡处理，通过培养法评价其杀菌效果。将使用过的外科口罩用MA-T喷洒或浸泡，然后进行培养以验证杀菌效果。在应用MA-T之前和之后评估N95口罩的性能。在喷洒MA-T后，与对照样品相比，人工污染的口罩和使用过的口罩的细菌数量大幅减少（分别为不适用和p =0.002）。MA-T浸泡后，两种口罩（人为污染的口罩和使用过的口罩）的细菌计数均显著降低（均p= 0.002）。对人工污染的大肠杆菌进行消毒试验。大肠杆菌和使用过的外科口罩表明，口罩可以分别采用MA-T喷雾消毒和浸泡消毒。在MA-T中浸泡30分钟后的N95口罩性能测试表明，MA-T对口罩进行了消毒，而没有降低口罩的性能。总之，MA-T是有用的口罩的重复使用，因为它的去污效果和安全性，同时保持口罩的功能。
Matching transformation system (MA-T), an on-demand aqueous chlorine dioxide solution, is an excellent safety disinfectant, because chlorine dioxide is not detected during storage or before use. The production of chlorine dioxide in MA-T is induced by a catalytic reaction in the presence of target microorganisms. In this study, we investigated MA-T disinfection of masks as a reuse method to eliminate mask shortages. After spraying Escherichia con on sterilized surgical mask, samples (factitiously contaminated masks) were treated with MA-T spraying or immersion, and the bactericidal efficacy was assessed by culturing. Used surgical masks were also sprayed with MA-T or were immersed in MA-T, and then were cultured to verify the bactericidal effect. The performance of N95 masks was assessed before and after application of MA-T. After spraying with MA-T, the numbers of bacteria of factitiously contaminated masks and used masks were drastically reduced compared with control samples (not applicable and p =0.002, respectively). After MA-T immersion, the bacterial counts of both masks (factitiously contaminated masks and used masks) were significantly reduced (both p= 0.002). Taken together, the disinfection test on factitiously contaminated with E. coli and used surgical masks showed that masks can be disinfected by MA-T spray and sterilized by immersion, respectively. The N95 mask performance test after 30min of immersion in MA-T showed that MA-T disinfected the mask without degrading the performance of the mask. In conclusion, MA-T is useful for the reuse of masks because of its decontamination effect and safety while maintaining the function of the mask.