Bovine Viral Diarrhea Virus Infection Induces Autophagy in MDBK Cells
Bovine Viral Diarrhea Virus Infection Induces Autophagy in MDBK Cells
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牛病毒性腹泻病毒感染诱导 MDBK 细胞自噬
DOI：
10.1007/s12275-014-3479-4
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发表时间：
2014-07-01
影响因子：
3
通讯作者：
Chen, Chuangfu
中科院分区：
文献类型：
--
作者：
Fu, Qiang;Shi, Huijun;Chen, Chuangfu
牛病毒性腹泻病毒（BVDV）是黄病毒科瘟病毒属的一种有包膜、正义单链RNA病毒。在感染BVDV的Madin-Darby牛肾（MDBK）细胞中，信号传导途径和信号分子水平发生改变。自噬是一种保守的生物降解途径，主要消除和降解受损或多余的细胞器和大分子复合物，用于真核细胞内的再循环。自噬也可以被诱导作为响应于不同应激（例如营养或生长因子剥夺、缺氧、活性氧物质暴露和病原体感染）维持细胞稳态的有效应答。然而，BVDV感染对MDBK细胞自噬的影响尚不清楚。因此，我们使用实时PCR、电子显微镜、激光共聚焦显微镜和Western印迹分析对BVDV NADL感染后的自噬活性进行了分析。结果表明，BVDV NADL感染增加了MDBK细胞中的自噬活性，并显著提高了自噬相关基因Beclin 1和ATG 14的表达水平。然而，通过RNA干扰（RNAi）敲低Beclin 1和ATG 14不影响BVDV NADL感染相关的自噬活性。这些发现为阐明病毒感染对宿主细胞的影响提供了新的视角。
Bovine viral diarrhea virus (BVDV) is an enveloped, positive-sense, single-stranded RNA virus that belongs to the genusPestivirus(Flaviviridae). The signaling pathways and levels of signaling molecules are altered in Madin-Darby Bovine Kidney (MDBK) cells infected with BVDV. Autophagy is a conservative biological degradation pathway that mainly eliminates and degrades damaged or superfluous organelles and macromolecular complexes for intracellular recycling in eukaryotic cells. Autophagy can also be induced as an effective response to maintain cellular homeostasis in response to different stresses, such as nutrient or growth factor deprivation, hypoxia, reactive oxygen species exposure and pathogen infection. However, the effects of BVDV infection on autophagy inMDBK cells remain unclear. Therefore, we performed an analysis of autophagic activity after BVDV NADL infection using real-time PCR, electron microscopy, laser confocal microscopy, and Western blotting analysis. The results demonstrated that BVDV NADL infection increased autophagic activity and significantly elevated the expression levels of the autophagy-related genes Beclin1 and ATG14 inMDBK cells. However, the knockdown of Beclin1 and ATG14 by RNA interference (RNAi) did not affect BVDV NADL infection-related autophagic activity. These findings provided a novel perspective to elaborate the effects of viral infection on the host cells.