Identification of regulatory RNA in bacterial genomes by genome-scale mapping of transcription start sites.
Identification of regulatory RNA in bacterial genomes by genome-scale mapping of transcription start sites.
复制标题
通过转录起始位点的基因组规模作图来鉴定细菌基因组中的调节 RNA。
DOI：
10.1007/978-1-62703-730-3_1
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Wade,JosephT
中科院分区：
文献类型：
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作者：
Singh,Navjot;Wade,JosephT
绘制转录起始位点的能力对于基因调控的研究和新RNA的鉴定至关重要。由于低覆盖率和/或RNA加工事件，常规RNA-seq通常不足以鉴定转录起始位点。我们已经开发了一种高灵敏度，基因组规模的方法，用于检测细菌中的转录起始位点。该方法使用cDNA文库的深度测序以在核苷酸分辨率下鉴定具有链特异性的转录起始位点。本文介绍了该方法在大肠杆菌转录起始位点鉴定中的应用。
The ability to map transcription start sites is critical for studies of gene regulation and for identification of novel RNAs. Conventional RNA-seq is often insufficient for identification of transcription start sites due to low coverage and/or RNA processing events. We have developed a highly sensitive, genome-scale method for detection of transcription start sites in bacteria. This method uses deep sequencing of cDNA libraries to identify transcription start sites with strand specificity at nucleotide resolution. Here, we describe the application of this method for transcription start site identification inEscherichia coli.