Determination of gamma-hydroxybutyrate (GHB), beta-hydroxybutyrate (BHB), pregabalin, 1,4-butane-diol (1,4BD) and gamma-butyrolactone (GBL) in whole blood and urine samples by UPLC-MSMS
Determination of gamma-hydroxybutyrate (GHB), beta-hydroxybutyrate (BHB), pregabalin, 1,4-butane-diol (1,4BD) and gamma-butyrolactone (GBL) in whole blood and urine samples by UPLC-MSMS
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DOI：
10.1016/j.jchromb.2011.12.009
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发表时间：
2012-02-15
影响因子：
3
通讯作者：
Strand, Dag Helge
中科院分区：
文献类型：
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作者：
Dahl, Sandra Rinne;Olsen, Kirsten Midtboen;Strand, Dag Helge
对γ-羟基丁酸酯（GHB）及其前体γ-丁内酯（GBL）和1，4-丁二醇（1，4-BD）以及普瑞巴林的高通量测定方法的需求正在增加。在这里，我们提出了两种分析方法，使用超高压液相色谱（UPLC）和串联质谱（MS/MS）检测GHB，β-羟基丁酸（BHB），普瑞巴林，1，4 BD和GBL在全血和尿液中的测定。使用96孔甲酸盐，全血方法是一种简单的高通量方法，适用于大样本量的筛选。该方法尿液样品制备简单，仅包括样品的稀释和过滤，适合尿液样品的快速筛查。两种方法均显示出可接受的线性、可接受的检测限和定量限。所有分析物的日内和日间精密度均低于10%RSD。从基质中提取分析物，回收率接近100%，未观察到主要基质效应。自2010年1月以来，这两种方法已被用作全血和尿液样本的常规筛查分析。(C)2011 Elsevier B. V.保留所有权利。
The demand of high throughput methods for the determination of gamma-hydroxybutyrate (GHB) and its precursors gamma-butyrolactone (GBL) and 1,4-butane-diol (1,4BD) as well as for pregabalin is increasing. Here we present two analytical methods using ultra-high pressure liquid chromatography (UPLC) and tandem mass spectrometric (MS/MS) detection for the determination of GHB, beta-hydroxybutyrate (BHB), pregabalin, 1,4BD and GBL in whole blood and urine. Using the 96-well formate, the whole blood method is a simple high-throughput method suitable for screening of large sample amounts. With an easy sample preparation for urine including only dilution and filtration of the sample, the method is suitable for fast screening of urine samples. Both methods showed acceptable linearity, acceptable limits of detection, and limits of quantification. The within-day and between-day precisions of all analytes were lower than 10% RSD. The analytes were extracted from matrices with recoveries near 100%, and no major matrix effects were observed. Both methods have been used as routine screening analyses of whole blood and urine samples since January 2010. (C) 2011 Elsevier B.V. All rights reserved.