The 5′ untranslated region of fruA mRNA in Myxococcus xanthus positively regulates the expression of its own gene
The 5′ untranslated region of fruA mRNA in Myxococcus xanthus positively regulates the expression of its own gene
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DOI：
10.1360/972004-227
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发表时间：
2004-11-01
影响因子：
--
通讯作者：
Mao, XH
中科院分区：
文献类型：
--
作者：
Wu, Q;Mao, XH
黄色粘球菌为研究多细胞形态发生机制提供了一个很好的模式生物。FruA是一种对黄瓜生长发育至关重要的转录因子，它的mRNA含有一个由235个核苷酸组成的很长的5‘-UTR。以lacZ为报告基因，构建了含有和缺失FruA 5‘-UTR的FruA-LacZ翻译融合基因，并分别整合在黄瓜染色体的Mx8噬菌体连接位点(AttB)上。在发育过程中，5‘-UTR从+4到+220之间的缺失取消了FruA-LacZ的表达，这表明5’-UTR在FruA的诱导中是必不可少的。对5‘-UTRRNA二级结构的预测表明，该区域可以形成一个非常稳定的三螺旋连接结构，它可能是调节蛋白的结合部位，也可能含有调控FruA表达的顺式作用元件(S)。因此，FruA基因的5‘-UTR正向调节其自身基因的表达。
Myxococcus xanthus provides an excellent model organism for studying the mechanism of multicellular morphogenesis. The mRNA for FruA, a transcription factor essential for the development of M. xanthus, contains a very long 5'-UTR consisting of 235 nucleotides. Using lacZ as a reporter gene, two fruA-lacZ translational fusions retaining or lacking the fruA 5'-UTR were constructed and separately integrated at phage Mx8 attachment site (attB) in M. xanthus chromosome. Deletion in 5'-UTR between nucleotides from +4 to +220 abolished fruA-lacZ expression during development, indicating that the 5'-UTR is essential for the induction of fruA. Prediction of the RNA secondary structure of 5'-UTR shows that this region could form an extremely stable three-helix junction structure, which might be a binding site for a regulatory protein or contain a cis-acting element(s) to control fruA expression. Thus, the 5'-UTR of fruA mRNA positively regulates the expression of its own gene.