Analysis of ascarosides from Caenorhabditis elegans using mass spectrometry and NMR spectroscopy.
Analysis of ascarosides from Caenorhabditis elegans using mass spectrometry and NMR spectroscopy.
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DOI：
10.1007/978-1-62703-619-1_6
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Butcher, Rebecca A
中科院分区：
文献类型：
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作者：
Zhang, Xinxing;Noguez, Jaime H;Zhou, Yue;Butcher, Rebecca A
线虫秀丽隐杆线虫分泌一个家庭的水溶性小分子，被称为ascarosides，进入其环境，并使用这些ascarosides在化学通讯。Ascarosides是3，6-双脱氧糖Ascarylose的衍生物，用不同的脂肪酸衍生的侧链修饰。C.秀丽线虫使用特定的子囊苷（它们一起被称为雌虫信息素）来触发进入抗应激的雌虫幼虫阶段。此外，C.秀丽线虫使用特定的蛔虫苷来控制某些行为，包括交配吸引、聚集和回避。虽然一般来说，环境中的浓度随着人口密度的增加而增加，但C。秀丽线虫可以根据其生长的培养条件和其发育历史改变其分泌的蛔虫苷的类型和量。在这里，我们描述了如何生长高密度蠕虫文化和这些文化的细菌食物，以及如何提取培养基产生粗信息素提取物。然后，我们讨论了如何使用质谱和核磁共振光谱分析该提取物中的蛔虫苷的类型和数量。
The nematode Caenorhabditis elegans secretes a family of water-soluble small molecules, known as the ascarosides, into its environment and uses these ascarosides in chemical communication. The ascarosides are derivatives of the 3,6-dideoxysugar ascarylose, modified with different fatty acid-derived side chains. C. elegans uses specific ascarosides, which are together known as the dauer pheromone, to trigger entry into the stress-resistant dauer larval stage. In addition, C. elegans uses specific ascarosides to control certain behaviors, including mating attraction, aggregation, and avoidance. Although in general the concentration of the ascarosides in the environment increases with population density, C. elegans can vary the types and amounts of ascarosides that it secretes depending on the culture conditions under which it has been grown and its developmental history. Here, we describe how to grow high-density worm cultures and the bacterial food for those cultures, as well as how to extract the culture medium to generate a crude pheromone extract. Then, we discuss how to analyze the types and amounts of ascarosides in that extract using mass spectrometry and NMR spectroscopy.