Comparative Transcriptomic and Lipidomic Analyses of Human Male and Female Meibomian Glands Reveal Common Signature Genes of Meibogenesis
Comparative Transcriptomic and Lipidomic Analyses of Human Male and Female Meibomian Glands Reveal Common Signature Genes of Meibogenesis
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DOI：
10.3390/ijms20184539
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发表时间：
2019-09-02
影响因子：
5.6
通讯作者：
Wojtowicz, Jadwiga C.
中科院分区：
文献类型：
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作者：
Butovich, Igor A.;Bhat, Nita;Wojtowicz, Jadwiga C.
眉板腺是由全分泌型眉板腺(MGs)分泌的一种脂类分泌物。MG是一种特殊类型的皮脂腺，嵌入在人的眼皮中。从化学上讲，蜂蜜和皮脂是不同的。需要对MG的脂质体和转录组进行详细的表征，以解开一系列复杂的和特征不佳的生物合成反应(称为Meibo发生)，这些反应导致meiban的形成。甲苯磺酸的组成和质量的变化与各种眼病有关，其中一些在男性中更常见，而另一些在女性中更常见。为了确定性别在人类小胚胎发育中的作用，我们对女性和男性的MG转录本和脂体进行了鉴定，并确定了两性小胚胎发育的特征基因。对MG组织标本进行基因芯片分析。用气相色谱和质谱仪对样品的化学成分进行了分析。采用了定向和非定向两种方法。已鉴定出约290个小核发育的特征基因。对它们的表达模式的分析表明，性别之间没有重大差异。主要种类的眉毛脂，如蜡酯、胆固醇酯、游离胆固醇、(O)-酰化欧米伽-羟基脂肪酸(OAHFA)、OAHFA的胆固醇酯和三酰甘油的脂谱也显示这些脂类只有很小的(和随机的)差异。转录分析的结果与脂体学数据有很好的相关性。综上所述，我们的数据表明，在男性和女性中，减数分裂以类似的方式进行，产生的分泌物成分相似，高度保守。这一发现对于设计针对各种MG相关疾病和病理情况的新的、独立于性别的诊断和治疗方法是重要的。
Meibum is a lipid secretion that is produced by holocrine Meibomian glands (MGs). MGs are a specialized type of sebaceous glands that are embedded in the human eyelids. Chemically, meibum and sebum are different. A detailed characterization of lipidome and transcriptome of MG is required to deconvolute a complex and poorly characterized array of biosynthetic reactions (termed meibogenesis) that lead to formation of meibum. Changes in the composition and quality of meibum have been linked to various ocular disorders, some of which are more prevalent in males, while others in females. To establish the role of gender in meibogenesis in humans, we characterized MG transcriptomes and lipidomes of females and males, and identified signature genes of meibogenesis in both genders. Specimens of MG tissues were subjected to mRNA microarray analyses. Chemical composition of meibum samples was assessed chromatographically and mass spectrometrically. Both targeted and untargeted approaches were used. About 290 signature genes of meibogenesis were identified. The analyses of their expression patterns demonstrated no major differences between the genders. Lipid profiling of major classes of meibomian lipids, such as wax esters, cholesteryl esters, free cholesterol, (O)-acylated omega-hydroxy fatty acids (OAHFA), cholesteryl esters of OAHFA, and triacylglycerols, also demonstrated only minor (and random) differences in these lipids. The results of transcriptomic analyses correlated well with lipidomic data. Taken together, our data imply that in males and females, meibogenesis proceeds in a similar fashion, yielding secretions with similar, highly conserved, compositions. This finding is important for designing novel, gender-independent diagnostic and therapeutic approaches to various MG-related diseases and pathological conditions.