Surface topography enhances differentiation of mesenchymal stem cells towards osteogenic and adipogenic lineages
Surface topography enhances differentiation of mesenchymal stem cells towards osteogenic and adipogenic lineages
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DOI：
10.1016/j.biomaterials.2015.05.030
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发表时间：
2015-08-01
期刊：
影响因子：
14
通讯作者：
Wagner, Wolfgang
中科院分区：
文献类型：
--
作者：
Abagnale, Giulio;Steger, Michael;Wagner, Wolfgang
表面形貌对细胞的生长和分化有影响，但要产生明确的表面结构并评估特定地形参数的相关性并不是一件容易的事。在这项研究中，我们系统地比较了间充质干细胞(MSCs)在不同的沟/脊结构上的体外分化。利用反应离子刻蚀和多光束激光干涉分别在聚酰亚胺中形成了微米和纳米图形。这些结构影响了人MSCs的细胞扩散和定向，这也反映在局灶性粘连的形态和大小上。时间推移显示出平行于纳米图案的定向迁移。总体而言，表面模式明显促进了MSCs向特定谱系的分化：15克骨脊促进成脂分化，而2克骨脊促进成骨分化。值得注意的是，周期为650 nm的纳米图案增加了对成骨和成脂谱系的分化。然而，在没有分化的情况下，培养基表面结构既没有诱导分化，也没有谱系特异性的基因表达变化。此外，纳米结构不影响YAP/TAZ复合体，该复合体由衬底硬度激活。我们的结果进一步揭示了定制生物材料和植入物界面的结构--例如通过亚微米级的多光束激光干涉--如何不能诱导MSCs本身分化，但支持它们的定向分化。(C)2015爱思唯尔有限公司。保留所有权利。
Surface topography impacts on cell growth and differentiation, but it is not trivial to generate defined surface structures and to assess the relevance of specific topographic parameters. In this study, we have systematically compared in vitro differentiation of mesenchymal stem cells (MSCs) on a variety of groove/ridge structures. Micro- and nano-patterns were generated in polyimide using reactive ion etching or multi beam laser interference, respectively. These structures affected cell spreading and orientation of human MSCs, which was also reflected in focal adhesions morphology and size. Time-lapse demonstrated directed migration parallel to the nano-patterns. Overall, surface patterns clearly enhanced differentiation of MSCs towards specific lineages: 15 gm ridges increased adipogenic differentiation whereas 2 gm ridges enhanced osteogenic differentiation. Notably, nano-patterns with a periodicity of 650 nm increased differentiation towards both osteogenic and adipogenic lineages. However, in absence of differentiation media surface structures did neither induce differentiation, nor lineage-specific gene expression changes. Furthermore, nanostructures did not affect the YAP/TAZ complex, which is activated by substrate stiffness. Our results provide further insight into how structuring of tailored biomaterials and implant interfaces - e.g. by multi beam laser interference in sub-micrometer scale do not induce differentiation of MSCs per se, but support their directed differentiation. (C) 2015 Elsevier Ltd. All rights reserved.