Expression and immunogenicity of novel subunit enterovirus 71 VP1 antigens.
Expression and immunogenicity of novel subunit enterovirus 71 VP1 antigens.
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DOI：
10.1016/j.bbrc.2012.03.067
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发表时间：
2012-04
影响因子：
3.1
通讯作者：
Juan Xu;Shixia Wang;Weihua Gan;Wenhong Zhang;L. Ju;Zu-hu Huang;Shan Lu
中科院分区：
文献类型：
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作者：
Juan Xu;Shixia Wang;Weihua Gan;Wenhong Zhang;L. Ju;Zu-hu Huang;Shan Lu
手足口病（HFMD）是一种常见于幼儿的病毒性疾病。手足口病是由属于小核糖核酸病毒科肠道病毒属的病毒引起的。最近，肠道病毒71（EV71）已成为手足口病的一种致病因子，具有严重的临床结局。在本报告中，我们进行了一项初步的抗原工程研究，以优化表达和免疫原性的亚单位VP1抗原的EV71疫苗的设计。采用DNA免疫作为一种简单的技术方法来测试不同设计的VP1抗原，而不需要首先在体外表达VP1蛋白。我们的研究表明，VP1蛋白的表达和免疫原性可以通过交替的VP1抗原设计来提高。本报告中提供的数据揭示了优化基于VP1抗原的EV71疫苗设计的新途径。
Hand, foot, and mouth disease (HFMD) is a common viral illness in young children. HFMD is caused by viruses belonging to the enterovirus genus of the picornavirus family. Recently, enterovirus 71 (EV71) has emerged as a virulent agent for HFMD with severe clinical outcomes. In the current report, we conducted a pilot antigen engineering study to optimize the expression and immunogenicity of subunit VP1 antigen for the design of EV71 vaccines. DNA immunization was adopted as a simple technical approach to test different designs of VP1 antigens without the need to express VP1 protein in vitro first. Our studies indicated that the expression and immunogenicity of VP1 protein can be improved with alternated VP1 antigen designs. Data presented in the current report revealed novel pathways to optimize the design of VP1 antigen-based EV71 vaccines.