Biotechnological production of acetoin, a bio-based platform chemical, from a lignocellulosic resource by metabolically engineered Enterobacter cloacae
Biotechnological production of acetoin, a bio-based platform chemical, from a lignocellulosic resource by metabolically engineered Enterobacter cloacae
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通过代谢工程阴沟肠杆菌从木质纤维素资源中生物技术生产乙偶姻（一种生物基平台化学品）
DOI：
10.1039/c5gc01638j
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发表时间：
2016-01-01
期刊：
影响因子：
9.8
通讯作者：
Ma, Cuiqing
中科院分区：
文献类型：
--
作者：
Zhang, Lijie;Liu, Qiuyuan;Ma, Cuiqing
乙偶姻（AC）被视为最具潜力的糖衍生化学结构单元之一，可用作食品添加剂、化学合成的前体以及植物生长促进分子。在本研究中，一种经过预处理的低成本木质纤维素资源——玉米秸秆被用作碳源来生产乙偶姻。在对阴沟肠杆菌SDM的代谢通量进行重定向、对还原力进行微调以及消除碳代谢物阻遏之后，构建了一个系统工程菌株SDM 53，它能够高效且同时利用葡萄糖和木糖。利用SDM 53进行补料分批发酵，使用木质纤维素水解物以1.52 g/L·h⁻¹的速率生产了45.6 g/L的乙偶姻。乙偶姻的生物技术合成具有多种优势，例如可持续且环境友好。凭借其理想的特性，工程菌株SDM 53可能是乙偶姻工业化生产的一个潜在选择。
Acetoin (AC) is regarded as one of the top potential sugar-derived chemical building blocks that can be used as food additives, precursors in chemical synthesis, and plant growth promoting molecules. In this study, a low-cost lignocellulosic resource of pretreated corn stover was used as a carbon source to produce AC. After redirecting the metabolic flux, fine tuning reducing power, and eliminating carbon catabolite repression in Enterobacter cloacae SDM, a systematically engineered strain SDM 53 was constructed, which is able to utilize glucose and xylose efficiently and simultaneously. Using fed-batch fermentation of SDM 53, 45.6 g L−1 AC was produced at a rate of 1.52 g L−1 h−1 using the lignocellulosic hydrolysate. Biotechnological synthesis of AC has various advantages such as being sustainable and environment-friendly. With its desirable properties, the engineered strain SDM 53 may be a potential choice for the industrial production of AC.