Exopolysaccharides produced by Bifidobacterium longum IPLA E44 and Bifidobacterium animalis subsp lactis IPLA R1 modify the composition and metabolic activity of human faecal microbiota in pH-controlled batch cultures
Exopolysaccharides produced by Bifidobacterium longum IPLA E44 and Bifidobacterium animalis subsp lactis IPLA R1 modify the composition and metabolic activity of human faecal microbiota in pH-controlled batch cultures
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DOI：
10.1016/j.ijfoodmicro.2009.08.017
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发表时间：
2009-11-15
影响因子：
5.4
通讯作者：
de los Reyes-Gavilan, Clara G.
中科院分区：
文献类型：
--
作者：
Salazar, Nuria;Ruas-Madiedo, Patricia;de los Reyes-Gavilan, Clara G.
从两种双歧杆菌菌株中分离出胞外多糖（EPS），其中一种是人肠道来源的双歧杆菌（长双歧杆菌亚种）。longum IPLA E44）和来自乳制品来源的另一种（动物双歧杆菌（Bifidobacterium animalis subsp. IPLA R1）进行体外化学模拟胃肠消化。这表明在这些条件下两种聚合物都没有降解。然后，聚合物被用作碳源的pH值控制的粪便分批培养，并与非益生元碳水化合物葡萄糖和益生元菊粉，以确定粪便细菌的组成的变化。一组八个荧光原位杂交寡核苷酸探针靶向16 S rRNA序列被用来量化特定的微生物组。条件致病菌溶组织梭菌的生长与所有检测的碳水化合物相似，与未添加碳水化合物的阴性对照培养物相似，主要归因于使用的培养条件，而不是这些底物对生长的促进作用。聚合物E44和RI以与菊粉类似的方式刺激乳杆菌/肠球菌、双歧杆菌和拟杆菌/普雷沃氏菌的生长。EPS RI还促进了在发酵的第一个24小时期间的Atopobium簇的生长。在发酵的早期阶段（第一个10-24小时）发现乙酸和乳酸的增加与乳酸杆菌，双歧杆菌，和Atopobium的增加。丙酸浓度增加，在旧的文化，这是一致的富集梭菌簇IX的培养物与EPS RI和拟杆菌的增加与微生物EPS（RI和E44）和菊粉的培养物。对于EPS E44获得最低的乙酸与丙酸比率。测试的碳水化合物均不支持来自梭菌簇XIVa+B和IV的微生物的生长，结果与EPS存在下的丁酸盐产量差相关。因此，由来自乳制品和肠道来源的双歧杆菌合成的EPS可以在体外调节肠道微生物群，促进一些数量和代谢相关微生物种群的变化以及短链脂肪酸生产的变化。(C)2009 Elsevier B. V.保留所有权利。
Exopolysaccharides (EPS) isolated from two Bifidobacterium strains, one of human intestinal origin (Bifidobacterium longum subsp. longum IPLA E44) and the other from dairy origin (Bifidobacterium animalis subsp. lactis IPLA R1), were subjected to in vitro chemically simulated gastrointestinal digestion. which showed the absence of degradation of both polymers in these conditions. Polymers were then used as carbon sources in pH-controlled faecal batch cultures and compared with the non-prebiotic carbohydrate glucose and the prebiotic inulin to determine changes in the composition of faecal bacteria. A set of eight fluorescent in situ hybridisation oligonucleotide probes targeting 16S rRNA sequences was used to quantify specific groups of microorganisms. Growth of the opportunistic pathogen Clostridium histolyticum occurred with all carbohydrates tested similarly to that found in negative control cultures without added carbohydrate and was mainly attributed to the culture conditions used rather than enhancement of growth by these substrates. Polymers E44 and RI stimulated growth of Lactobacillus/Enterococcus, Bifidobacterium, and Bacteroides/Prevotella in a similar way to that seen with inulin. The EPS RI also promoted growth of the Atopobium cluster during the first 24 h of fermentation. An increase in acetic and lactic acids was found during early stages of fermentation (first 10-24 h) correlating with increases of Lactobacillus, Bifidobacterium, and Atopobium. Propionic acid concentrations increased in old cultures, which was coincident with the enrichment of Clostridium cluster IX in cultures with EPS RI and with the increases in Bacteroides in cultures with both microbial EPS (RI and E44) and inulin. The lowest acetic to propionic acid ratio was obtained for EPS E44. None of the carbohydrates tested supported the growth of microorganisms from Clostridium clusters XIVa+b and IV, results that correlate with the poor butyrate production in the presence of EPS. Thus, EPS synthesized by bifidobacteria from dairy and intestinal origins can modulate the intestinal microbiota in vitro, promoting changes in some numerically and metabolically relevant microbial populations and shifts in the production of short chain fatty acids. (C) 2009 Elsevier B.V. All rights reserved.