Measurement of blood-oxygen saturation using a photoacoustic technique in the rabbit hypoxemia model
Measurement of blood-oxygen saturation using a photoacoustic technique in the rabbit hypoxemia model
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DOI：
10.1007/s10877-018-0166-8
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发表时间：
2018-06
影响因子：
2.2
通讯作者：
K. Sei;M. Fujita;T. Hirasawa;S. Okawa;T. Kushibiki;H. Sasa;K. Furuya;M. Ishihara
中科院分区：
文献类型：
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作者：
K. Sei;M. Fujita;T. Hirasawa;S. Okawa;T. Kushibiki;H. Sasa;K. Furuya;M. Ishihara
准确获得血氧饱和度的金标准方法是血气分析，需要有创的采血过程。光声技术使我们能够无创地实时测量血氧饱和度。本研究的目的是使用光声技术，一种光学方法，用于准确地确定氧饱和度在体内。我们测量了在750和800 nm照射后稳定低氧血症的兔模型动脉血的诱导光声信号。使用两条校准曲线从光声信号计算氧饱和度。校准曲线1是从血红蛋白的吸收系数导出的常规曲线，而校准曲线2是从从原始血管模型获得的光声信号导出的。同时进行血气分析，以获得氧饱和度的参考标准。进行回归分析和Bland-Altman分析，以评估使用两种方法获得的血氧饱和度的准确性。使用校准曲线1和2计算的氧饱和度在回归分析中显示与参比标准品的强相关性（分别为R = 0.965、0.964）。Bland-Altman分析显示与校准曲线2的一致性和精密度更好，而使用校准曲线1获得的值存在显著低估。使用校准曲线2的氧饱和度的光声测量提供了氧饱和度的准确估计，其类似于使用便携式血气分析仪获得的氧饱和度。
The golden standard method to obtain accurate blood oxygen saturation is blood gas analysis that needs invasive procedure of blood sampling. Photoacoustic technique enables us to measure real-time blood oxygen saturation without invasive procedure. The aim of this study is to use the photoacoustic technique, an optical method, for accurately determining oxygen saturation in vivo. We measured induced photoacoustic signals of arterial blood in the rabbit model of stable hypoxemia after irradiation at 750 and 800 nm. Oxygen saturation was calculated from the photoacoustic signals using two calibration curves. Calibration curve 1 is a conventional curve derived from the absorbance coefficient of hemoglobin, whereas calibration curve 2 is derived from the photoacoustic signals obtained from the original blood vessel model. Simultaneously, blood-gas analysis was performed to obtain the reference standard of oxygen saturation. Regression analysis and Bland–Altman analysis were performed to assess the accuracy of oxygen saturation obtained using the two methods. The oxygen saturation calculated using calibration curves 1 and 2 showed strong correlations with the reference standard in regression analysis (R = 0.965, 0.964, respectively). The Bland–Altman analysis revealed better agreement and precision with calibration curve 2, whereas there was significant underestimation of values obtained using calibration curve 1. Photoacoustic measurement of oxygen saturation using calibration curve 2 provided an accurate estimate of oxygen saturation, which was similar to that obtained using a portable blood-gas analyzer.