Near-infrared fluorescence imaging of microcalcification in an animal model of breast cancer
Near-infrared fluorescence imaging of microcalcification in an animal model of breast cancer
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DOI：
10.1016/s1076-6332(03)00253-8
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发表时间：
2003-10-01
影响因子：
4.8
通讯作者：
Goldberg, SN
中科院分区：
文献类型：
--
作者：
Lenkinski, RE;Ahmed, M;Goldberg, SN
理由和目标。目前，还没有形成可重复微钙化的乳腺癌动物模型。本研究的目的是开发一个简单的，可重复的模型系统，可用于开发多模态造影剂，用于识别乳腺癌微钙化。将R3230乳腺癌细胞系植入雌性Fischer 344大鼠的乳腺脂肪垫中（两只大鼠具有两个植入的肿瘤，两只大鼠具有单个植入的肿瘤）。在生长至直径1- 2cm后，将100 μ m羟基磷灰石晶体（阳性对照）或草酸钙晶体（阴性对照）植入肿瘤。晶体植入后24小时，大鼠静脉注射一种以前描述的近红外荧光双膦酸盐衍生物Pam 78，并使用反射光学成像系统对肿瘤进行成像。植入羟基磷灰石的肿瘤在晶体植入区域显示明亮的局灶性近红外荧光。生长在同一动物体内并植入草酸钙的对照肿瘤，没有显示任何近红外荧光，甚至沿着用于晶体植入的针迹也没有显示。一种简单快速的乳腺癌肿瘤局灶性钙化动物模型已经开发和验证。该模型使用Pam 78，一种近红外荧光造影剂，专用于羟基磷灰石。潜在的有用性的模型开发类似的造影剂的磁共振和其他成像方式进行了讨论。
Rationale and Objectives. At present, there is no animal model of breast cancer that forms reproducible microcalcification. The aim of this study was to develop a straightforward, reproducible model system that could be used to develop multimodality contrast agents for the identification of breast cancer microcalcification.Methods. The R3230 mammary adenocarcinoma cell line was implanted in the mammary fat pad of female Fischer 344 rats (two rats with two implanted tumors and two rats with a single implanted tumor). After growth to 1-2 cm in diameter, tumors were implanted with 100 mum hydroxyapatite crystals (positive control) or calcium oxalate crystals (negative control). Twenty-four hours after crystal implantation, rats were injected intravenously with a previously described near-infrared fluorescent bisphosphonate derivative known as Pam78, and the tumors were imaged using a reflectance optical imaging system.Results. Tumors implanted with hydroxyapatite displayed bright, focal, near-infrared fluorescence in the area of crystal implantation. Control tumors, grown in the same animal and implanted with calcium oxalate, did not display any near-infrared fluorescence, even along the needle track used for crystal implantation.Conclusions. A simple and rapid animal model of focal calcification in breast cancer tumors has been developed and validated. The model used Pam78, a near-infrared fluorescent contrast agent specific for hydroxyapatite. The potential usefulness of the model for developing similar contrast agents for magnetic resonance and other imaging modalities is discussed.