Relationship between VEGF and p53 expression and tumor cell proliferation in human gastrointestinal carcinomas
Relationship between VEGF and p53 expression and tumor cell proliferation in human gastrointestinal carcinomas
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DOI：
10.1007/s00432-007-0270-5
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发表时间：
2008-02-01
影响因子：
3.6
通讯作者：
Tonino, Paola
中科院分区：
文献类型：
--
作者：
Montero, Elvira;Abreu, Carmen;Tonino, Paola
目的血管内皮生长因子(VEGF)和P53在肿瘤生长中起重要作用。然而，人胃肠道肿瘤中血管内皮生长因子和p53的表达与肿瘤细胞增殖的关系尚不清楚。方法采用免疫组织化学方法检测30例胃肠道癌组织中血管内皮生长因子、p53蛋白和增殖细胞核抗原的表达。结果30例胃肠道癌组织中有16例(54.84%)以中等强度表达为主，而P53蛋白主要为高表达13例(45.16%)。30例GITC中有20例(64.52%)呈高表达。肿瘤大小、浸润性、血管侵犯和胃炎与血管内皮生长因子、p53和增殖细胞核抗原的表达显著相关。血管内皮生长因子与P53表达(P=0.0001)、血管内皮生长因子与增殖细胞核抗原表达(P=0.00004)、P53表达与增殖细胞核抗原表达(P=0.0016)呈显著正相关。当同时考虑VEGF和P53的表达以及增殖细胞核抗原的表达时，两者的表达与增殖细胞核抗原的表达均无显著相关性。结论血管内皮生长因子和P53蛋白的表达与肿瘤细胞增殖密切相关，可能与胃肠道肿瘤生物学相关事件有关。
Purpose The vascular endothelial growth factor (VEGF) and p53 play important roles in the growth of tumor. However, the relationship between the expression of VEGF and p53 and tumor cell proliferation in human gastrointestinal cancer remains unknown. In the present study, therefore, we have examined the relationship between VEGF and p53 expression and tumor cell proliferation in gastrointestinal carcinoma (GITC), as well as the association between these biomarkers and clinicopathological factors.Methods Surgical specimens from 30 patients with GITC were examined for VEGF, p53, and proliferating cell nuclear antigen (PCNA) expression by immunohistochemical staining.Results We found a predominant VEGF expression of moderate intensity in 16(54.84%) of 30 GITC cases, while p53 expression was mainly high in 13(45.16%) of 30 GITC cases. PCNA expression was high in 20(64.52%) of 30 GITC cases. Tumor size, infiltration, vascular invasion, and gastritis were significantly correlated with VEGF, p53, and PCNA expression. There was a significant correlation between VEGF and p53 expression (P = 0.0001), VEGF and PCNA expression (P = 0.00004), and between p53 expression and PCNA expression (P = 0.0016). When the VEGF and p53 expression, and PCNA expression were considered together, both VEGF and p53 expression were not significantly associated with PCNA. A significant correlation between the PCNA expression and the mitotic index (P = 0.0016) was also found.Conclusion These results demonstrate that VEGF and p53 expression are significantly correlated as independent prognostic factors with tumor cell proliferation, and might be associated with relevant events involved in gastrointestinal tumor biology.