The MADS-domain protein MPF1 of Physalis floridana controls plant architecture, seed development and flowering time
The MADS-domain protein MPF1 of Physalis floridana controls plant architecture, seed development and flowering time
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DOI：
10.1007/s00425-009-1087-z
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发表时间：
2010-02-01
期刊：
影响因子：
4.3
通讯作者：
Saedler, Heinz
中科院分区：
文献类型：
--
作者：
He, Chaoying;Tian, Ying;Saedler, Heinz
植物的花和营养发育依赖于MADS结构域转录因子的组合作用。STMADS11亚支的成员，如酸浆属的MPF1，在叶片和花器官中大量表达，但其功能尚不清楚。我们对过表达MPF1的转基因拟南芥的研究表明，MPF1与SOC1相互作用决定开花时间。然而，MPF1 RNAi介导的酸浆植株表现出复杂的表型，其开花时间、植株结构和种子大小都发生了变化。与野生型相比，这些植物的开花时间推迟了约20%。在MPF1 RNAi细胞系中，PFLFY的表达上调，而pfft和MPF3基因的表达受到强烈抑制。MPF1与MADS结构域因子的一个子集相互作用，即在植物中与PFSOC1相互作用，在酵母中与PFSEP3和PFFUL相互作用，支持该蛋白在开花过程中的调节作用。转基因MPF1 RNAi植株产生的种子平均大小增加了近两倍。这些植物的高度也增加了大约两倍，但与对照相比，大多数腋芽都发育不良。综上所述，这表明STMADS11亚类成员对开花具有积极的调节作用，但在植物生长中具有不同的功能。
Floral and vegetative development of plants is dependent on the combinatorial action of MADS-domain transcription factors. Members of the STMADS11 subclade, such as MPF1 of Physalis, are abundantly expressed in leaves as well as in floral organs, but their function is not yet clear. Our studies with transgenic Arabidopsis that over-express MPF1 suggest that MPF1 interacts with SOC1 to determine flowering time. However, MPF1 RNAi-mediated knockdown Physalis plants revealed a complex phenotype with changes in flowering time, plant architecture and seed size. Flowering of these plants was delayed by about 20% as compared to wild type. Expression of PFLFY is upregulated in the MPF1 RNAi lines, while PFFT and MPF3 genes are strongly repressed. MPF1 interacts with a subset of MADS-domain factors, namely with PFSOC1 in planta, and with PFSEP3 and PFFUL in yeast, supporting a regulatory role for this protein in flowering. The average size of seeds produced by the transgenic MPF1 RNAi plants is increased almost twofold. The height of these plants is also increased about twofold, but most axillary buds are stunted when compared to controls. Taken together, this suggests that members of the STMADS11 subclade act as positive regulators of flowering but have diverse functions in plant growth.