Protective efficacy of an inactivated vaccine against H9N2 avian influenza virus in ducks.
Protective efficacy of an inactivated vaccine against H9N2 avian influenza virus in ducks.
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H9N2禽流感病毒灭活疫苗对鸭的保护效果
DOI：
10.1186/s12985-015-0372-7
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发表时间：
2015-09-17
期刊：
影响因子：
4.8
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Teng Q;Shen W;Liu Q;Rong G;Chen L;Li X;Chen H;Yang J;Li Z
野鸭在禽流感病毒(AIV)的进化过程中起着重要作用。众所周知，中国的家鸭携带和传播H9N2型禽流感病毒，这种病毒被认为是导致最近爆发的人畜共患病H7N9病毒的内部基因。为了保护动物和公众健康，迫切需要一种有效的疫苗来阻断和防止H9N2病毒在鸭体内的传播。我们以流行的H9N2型禽流感病毒为基础，研制了一种H9N2灭活疫苗(含Montanide ISA 70VG佐剂)，并在鸭身上进行了评估。结果表明，该灭活疫苗能够诱导免疫鸭产生强烈而快速的体液免疫应答。血清中血凝抑制效价迅速升高，免疫后5周达到最高值12.3log2，免疫后至少37周仍保持在较高水平。免疫后3周和37周，用同源H9N2AIV免疫鸭可完全阻断病毒的脱落。结论H9N2AIV灭活疫苗能诱导鸭产生高效、持久的免疫应答，能有效地保护鸭免受H9N2病毒感染。
BackgroundWild ducks play an important role in the evolution of avian influenza viruses (AIVs). Domestic ducks in China are known to carry and spread H9N2 AIVs that are thought to have contributed internal genes for the recent outbreak of zoonotic H7N9 virus. In order to protect animal and public health, an effective vaccine is urgently needed to block and prevent the spread of H9N2 virus in ducks. We developed an inactivated H9N2 vaccine (with adjuvant Montanide ISA 70VG) based on an endemic H9N2 AIV and evaluated this vaccine in ducks.FindingsThe results showed that the inactivated H9N2 vaccine was able to induce a strong and fast humoral immune response in vaccinated ducks. The hemagglutination inhibition titer in the sera increased fast, and reached its peak of 12.3 log2 at 5 weeks post-vaccination in immunized birds and remained at a high level for at least 37 weeks post-vaccination. Moreover, viral shedding was completely blocked in vaccinated ducks after challenge with a homologous H9N2 AIV at both 3 and 37 weeks post-vaccination.ConclusionsThe results of this study indicate that the inactivated H9N2 vaccine induces high and prolonged immune response in vaccinated ducks and are efficacious in protecting ducks from H9N2 infection.