Attenuation of arsenic induced high fat diet exacerbated oxidative stress mediated hepatic and cardiac injuries in male Wistar rats by piperine involved antioxidative mechanisms
Attenuation of arsenic induced high fat diet exacerbated oxidative stress mediated hepatic and cardiac injuries in male Wistar rats by piperine involved antioxidative mechanisms
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DOI：
10.1016/j.fct.2020.111477
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发表时间：
2020-08-01
影响因子：
4.3
通讯作者：
Bandyopadhyay, Debasish
中科院分区：
文献类型：
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作者：
Dey, Tiyasa;Ghosh, Auroma;Bandyopadhyay, Debasish
本研究探讨胡椒碱对砷诱导的高脂饮食加重氧化应激介导的雄性Wistar大鼠肝脏和心脏组织损伤的缓解作用。口服胡椒碱显著（p < 0.05）降低了砷和高脂日粮处理大鼠肝脏和心脏组织中器官特异性和氧化应激生物标志物的水平，并呈剂量依赖性，其中剂量为60 mg/kg体重时表现出最大的保护作用。砷诱导的高脂饮食加重了氧化应激介导的肝脏和心脏组织损伤，导致抗氧化酶活性降低，ROS生成，克雷布斯循环和呼吸链酶活性降低，线粒体膜电位塌陷，线粒体DNA损伤，脂质代谢和炎症细胞因子水平改变。组织化学和组织病理学研究支持上述发现。胡椒碱通过调节抗氧化防御机制和自由基猝灭能力，有效地抵消了高脂饲料中砷引起的氧化应激损伤。这些发现表明，胡椒碱可以保护砷引起的高脂肪饮食加重的肝脏和心脏损伤，这强调了胡椒碱在改善砷引起的高脂肪饮食加重的氧化应激介导的器官损伤方面的重要性。
The current study explored the efficacy of piperine in attenuating arsenic induced high fat diet aggravated oxidative stress mediated injury in hepatic and cardiac tissues of male Wistar rats. Oral administration of piperine significantly (p < 0.05) reduced the levels of organ specific and oxidative stress biomarkers in arsenic and high fat diet treated rat hepatic and cardiac tissues in a dose dependant manner with the dose of 60 mg/kg b.w. exhibiting maximum protection. Arsenic induced high fat diet aggravated oxidative stress mediated damages in liver and heart tissues led to decreased activities of antioxidant enzymes, ROS generation, diminished activities of Krebs' cycle and respiratory chain enzymes, collapsed mitochondrial membrane potential, mitochondrial DNA damage along with altered lipid metabolism and inflammatory cytokine levels. Histochemical and histopathological studies supported the above findings. Piperine efficiently counteracted the arsenic induced high fat diet aggravated oxidative stress mediated damages by modulating antioxidant defense mechanism along with free radical quenching ability. These findings indicate that piperine protected the arsenic induced high fat diet aggravated hepatic and cardiac injuries which underline the importance of piperine in providing a possible therapeutic regime for the amelioration of arsenic-induced high fat diet aggravated oxidative stress mediated organ damages.