Biocontrol by yeasts of blue mould of citrus fruits and the mode of action of an isolate of Pichia guilliermondii
Biocontrol by yeasts of blue mould of citrus fruits and the mode of action of an isolate of Pichia guilliermondii
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DOI：
10.1080/14620316.1998.11510993
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发表时间：
1998
影响因子：
1.9
通讯作者：
G. Arras;V. Cicco;S. Arru;G. Lima
中科院分区：
文献类型：
--
作者：
G. Arras;V. Cicco;S. Arru;G. Lima
摘要从不同水果中分离了19株酵母菌，对人工损伤柑橘果实的蓝霉（青霉）拮抗活性进行了研究。与对照相比，吉氏毕赤酵母1a、5A、29A菌株，念珠菌21D、22D、30F菌株和清酒酵母13l菌株的感染率降低了86% ~ 98%。吉利蒙地弓形虫5a分离物是最有效的拮抗剂，并对其作用方式进行了进一步的研究。随着接种酵母细胞数量的减少，其抗病原菌活性下降，但其在创面上具有良好的定殖能力和快速生长能力，在24 h内细胞数量最多可增加60倍。创面扫描电镜也显示酵母细胞附着在病原菌菌丝上。分离物5A的高拮抗活性似乎与它占据伤口空间和利用伤口中可用营养物质的能力有关，从而剥夺了病原体的营养。它还诱发了……
SummaryNineteen yeast isolates, from different fruits, were tested for antagonistic activity against blue mould (Penicillium italicum) on artificially wounded citrus fruits. Strains 1 A, 5A, and 29A of Pichia guilliermondii, strains 21D, 22D and 30F of Candida famata and strain 13 L of C. sake reduced infection from 86 to 98% compared with the control. Isolate 5 A of P. guilliermondii was the most effective antagonist and its mode of action was investigated further. Its activity against the pathogen declined when the number of yeast cells inoculated in the wound was reduced although it showed good ability to colonize the wound site and grow rapidly, cell numbers increasing by up to 60-fold within 24 h. Scanning electron microscopy of the wound also showed attachment of the yeast cells to the pathogen hyphae. The high antagonistic activity of isolate 5A appeared to be related to its capability to occupy space and use nutrients available in the wound, thus depriving the pathogen of them. It also induced in ...