Electronic imaging system for direct and rapid quantitation of fluorescence from electrophoretic gels: application to ethidium bromide-stained DNA.
Electronic imaging system for direct and rapid quantitation of fluorescence from electrophoretic gels: application to ethidium bromide-stained DNA.
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DOI：
10.1016/0003-2697(87)90247-8
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发表时间：
1987-06
影响因子：
2.9
通讯作者：
John C. Sutherland;Bohai Lin;D. Monteleone;JoAnn H. Mugavero;B. M. Sutherland;J. Trunk
中科院分区：
文献类型：
--
作者：
John C. Sutherland;Bohai Lin;D. Monteleone;JoAnn H. Mugavero;B. M. Sutherland;J. Trunk
我们建立了一个基于改进的电荷耦合器件电视摄像机的电子成像系统，该系统可以直接定量电泳凝胶、色谱图和其他固定源的荧光分布。曝光时间可能超过 1 分钟。与它所取代的照相系统不同，相机的响应与入射荧光的强度成正比，并且图像数据被数字化并存储在计算机存储器中，以便在曝光完成后立即进行分析。我们描述了图像数据的显示、标准化和存档存储的程序，以及使用碱性琼脂糖凝胶中溴化乙锭染色的 DNA 图像来定量 DNA 中单链断裂的程序。
We have built an electronic imaging system based on a modified charge-coupled-device television camera that directly quantitates the distribution of fluorescence from electrophoretic gels, chromatograms, and other stationary sources. Exposure times can exceed 1 min. Unlike the photographic system that it replaces, the response of the camera is directly proportional to the intensity of incident fluorescence, and image data are digitized and stored in computer memory ready for analysis immediately upon completion of an exposure. We describe procedures for the display, normalization, and archival storage of image data and programs that use images of ethidium bromide-stained DNA in alkaline agarose gels to quantitate single-strand breaks in DNA.