GENERAL REQUIREMENT FOR RNA-POLYMERASE-II HOLOENZYMES IN-VIVO
GENERAL REQUIREMENT FOR RNA-POLYMERASE-II HOLOENZYMES IN-VIVO
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DOI：
10.1073/pnas.92.10.4587
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发表时间：
1995-05-09
影响因子：
11.1
通讯作者：
YOUNG, RA
中科院分区：
文献类型：
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作者：
THOMPSON, CM;YOUNG, RA
酵母RNA聚合酶II全酶已被描述为由RNA聚合酶II、一般转录因子的子集和九种SRB调节蛋白组成。区别RNA聚合酶II全酶从其他形式的RNA聚合酶II在细胞中的特征是它们与SRB蛋白的紧密关联，我们通过检查SRB基因中的温度敏感性突变对RNA聚合酶II转录的影响来研究体内需要SRB蛋白的基因的分数。转移到限制性温度后，srb突变细胞中的mRNA合成迅速和全面关闭。这些数据，结合细胞中基本上所有SRB蛋白与RNA聚合酶II分子紧密相关的观察，认为含SRB的全酶是RNA聚合酶II的形式，被招募到细胞中的大多数启动子。
Yeast RNA polymerase II holoenzymes have been described that consist of RNA polymerase II, a subset of general transcription factors, and nine SRB regulatory proteins. The feature that distinguishes the RNA polymerase II holoenzymes from other forms of RNA polymerase II in the cell is their tight association with SRB proteins, We investigated the fraction of genes that require SRB proteins in vivo by examining the effect of temperature-sensitive mutations in SRB genes on transcription by RNA polymerase II. Upon transfer to the restrictive temperature, there is a rapid and general shutdown of mRNA synthesis in srb mutant cells. These data, combined with the observation that essentially all of the SRB protein in cells is tightly associated with RNA polymerase II molecules, argue that SRB-containing holoenzymes are the form of RNA polymerase II recruited to most promoters in the cell.