Histoproliferative effect of Rauscher leukemia virus on lymphatic tissue: histological and ultrastructural studies of germinal centers and their relation to leukemogenesis.
Histoproliferative effect of Rauscher leukemia virus on lymphatic tissue: histological and ultrastructural studies of germinal centers and their relation to leukemogenesis.
复制标题
劳舍尔白血病病毒对淋巴组织的组织增殖作用：生发中心的组织学和超微结构研究及其与白血病发生的关系。
作者：
M. Hanna;A. Szakal;R. Tyndall
本文研究了成年BALB/c小鼠腹腔注射Rauscher病毒制剂后淋巴组织的早期组织学和超微结构变化。在注射后6小时至20天的时间间隔内研究脾脏、胸腺和肠系膜淋巴结。特别注意的是，在劳舍尔病毒诱导的白血病发生的早期阶段，在生发中心的变化。组织学上，劳舍尔病毒灭活注射小鼠中的独特变化是生发中心的增生，在24小时时检测到，并持续到中心的“分离性生长”。许多C型病毒被观察到位于细胞外的网状细胞的质膜内折叠，构成基质的中心。在注射Rauscher病毒制剂后4 - 7天，与最初在中心和最终在整个结节中增殖的细胞在形态上无法区分的细胞分散在整个脾红髓中。本研究的一个最重要的方面是在脾脏中识别参与C型颗粒复制的免疫母细胞。早在注射后24小时，生殖中心的实质免疫母细胞中的C型颗粒出芽。到第7天，在脾红髓中检测到与造血细胞密切相关的病毒复制免疫母细胞。有人建议，免疫母细胞可能，作为事件的顺序方面的结果，提供C型病毒的来源，脾红髓的增殖细胞，如巨核细胞和红细胞前体细胞。在本研究的后期间隔，还观察到红髓中这些细胞之间的Viropexis。一般而言，这些发现与生发中心基质网的抗原定位能力以及整体免疫抑制相关。
The early histological and ultrastructural changes in lymphatic tissue were studied in adult BALB/c mice which had received i.p. injections of Rauscher virus preparation. The spleen, thymus, and mesenteric lymph node were studied at intervals between 6 hr and 20 days after injection. Special attention was given to the changes in germinal centers during the early phases of the Rauscher virus-induced leukemogenesis. Histologically, the singular distinct alteration in the Rauscher virus preparation-injected mice was a hyperplasia in the germinal centers, detected at 24 hr and continuing into what is described as “dissociative growth” of the center. Numerous C-type viruses were observed to be localized extracellularly in the plasma membrane infoldings of reticular cells which constitute the stroma of the centers. Between 4 and 7 days after Rauscher virus preparation injection, cells morphologically indistinguishable from those originally seen proliferating in the centers and eventually in the entire nodule were dispersed throughout the spleen red pulp. A most important aspect of this study was the identification in the spleen of immunoblasts participating in the replication of C-type particles. Budding of C-type particles from parenchymal immunoblasts of the germinal centers occurred as early as 24 hr after injection. By 7 days, the virus-replicating immunoblasts were detected in the spleen red pulp in close association with hematopoietic cells. It was suggested that the immunoblasts may, as a result of the sequential aspects of the events, provide a source of C-type virus to proliferating cells of the spleen red pulp, such as megakaryocytes and erythrocytic precursor cells. Viropexis among these cells in the red pulp was also observed at the latter intervals of this study. In general, these findings correlate with the antigen-localizing capacity of the stromal reticulum of the germinal centers, as well as overall immune suppression.