15N/14N ratios of amino acids as a tool for studying terrestrial food webs: a case study of terrestrial insects (bees, wasps, and hornets)
15N/14N ratios of amino acids as a tool for studying terrestrial food webs: a case study of terrestrial insects (bees, wasps, and hornets)
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DOI：
10.1007/s11284-011-0844-1
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发表时间：
2011-07-01
影响因子：
2
通讯作者：
Ohkouchi, Naohiko
中科院分区：
文献类型：
--
作者：
Chikaraishi, Yoshito;Ogawa, Nanako O.;Ohkouchi, Naohiko
氨基酸的化合物特异性稳定同位素分析（CSIA）是一种新的方法，可以估计消费者在食物网中的营养地位。我们研究了日本社会性昆虫（三蜜蜂，三黄蜂，四大黄蜂种）的氨基酸的氮同位素组成（δ N-15），以评估潜在的CSIA的陆地食物网的研究中的氨基酸。对于黄蜂，我们还研究了不同生长阶段（从卵到成虫）的样品，以评估变态对CSIA估计营养位置的影响。日本社会性昆虫的散装组织的Δ N-15值与生物学预期的营养位置仅弱相关。相比之下，从氨基酸的Δ N-15值估计的营养位置（对于蜜蜂产生2.0和2.3之间的值，对于黄蜂产生2.8和3.3之间的值，对于黄蜂产生3.5和4.1之间的值）与这些昆虫的生物学预期营养位置一致（即，2.0蜜蜂3.0，黄蜂3.0，大黄蜂3.0 - 4.0）。虽然在单个氨基酸的Δ N-15值之间观察到较大的变异性（例如，例如，在一个实施例中，苯丙氨酸的含量为3.0 - 14.9ppm），但在黄蜂变态过程中，营养位置没有明显变化。因此，氨基酸的CSIA是一个强大的工具，不仅调查水生食物网，但也陆地捕食性昆虫的食物网。
Compound-specific stable isotope analysis (CSIA) of amino acids is a new method that enables estimates of trophic position for consumers in food webs. We examined the nitrogen isotopic composition (delta N-15) of amino acids of Japanese social insects (three bee, three wasp, and four hornet species) to evaluate the potential of CSIA of amino acids in studies of terrestrial food webs. For wasps, we also examined samples at different growth stages (ranging from egg to adult) to assess the effect of metamorphosis on CSIA estimates of trophic position. The delta N-15 values of bulk tissues for Japanese social insects are only weakly correlated with the biologically expected trophic positions. In contrast, the trophic positions estimated from the delta N-15 values of amino acids (yielding values of between 2.0 and 2.3 for bees, between 2.8 and 3.3 for wasps, and between 3.5 and 4.1 for hornets) are consistent with the biologically expected trophic positions for these insects (i.e., 2.0 for bees, 3.0 for wasps, and 3.0-4.0 for hornets). Although large variability is observed among the delta N-15 values of individual amino acids (e. g., ranging from 3.0 to 14.9 parts per thousand for phenylalanine), no significant change is observed in the trophic position during wasp metamorphosis. Thus, the CSIA of amino acids is a powerful tool for investigating not only aquatic food webs but also terrestrial food webs with predatory insects.