Changes in articular cartilage after intra-articular injections of methylprednisolone acetate in horses.
Changes in articular cartilage after intra-articular injections of methylprednisolone acetate in horses.
复制标题
马关节内注射醋酸甲泼尼龙后关节软骨的变化。
DOI：
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发表时间：
1989
影响因子：
1
通讯作者：
Maylin Ga
中科院分区：
文献类型：
--
作者：
S. Chunekamrai;Krook Lp;G. Lust;Maylin Ga
8匹没有关节疾病迹象或关节内治疗史的成熟马接受了每周一次的醋酸甲基强的松龙关节内注射。治疗剂量为120mg /关节，注射至右侧桡腕关节和腕间关节，左侧关节作为未治疗对照。末次注射后1、4、8周解剖关节软骨标本。与对照组相比，注射关节软骨嗜苏木精性丧失，红素O耐晒绿染色强度降低。所有治疗后的关节软骨样本均出现软骨细胞坏死和细胞增多。蛋白聚糖含量降低，合成速度减慢。蛋白多糖含量逐渐减少，而蛋白多糖合成在治疗后4周和8周有所增加。胶原蛋白含量不变，但其合成速率明显受到抑制。胶原合成没有恢复，但仍然减少了未处理软骨值的5%至15%。水分含量增加，但纤维连接蛋白含量差异不显著。本组8匹马中，3匹马右掌指关节单次注射醋酸甲基强的松龙，左关节作为未治疗对照。治疗16周后，治疗关节软骨组织化学染色消失，蛋白多糖含量降至对照组的50%。蛋白聚糖平均合成速率和纤维连接蛋白平均含量均升高，但差异无统计学意义（P > 0.05）。其他变量基本不变。（摘要删节250字）
Eight mature horses with no prior signs of joint disease or history of intra-articular therapy were treated with 8 weekly intra-articular injections of methylprednisolone acetate. Treatments were given at a dose of 120 mg/joint into the right radiocarpal and intercarpal joints, with the left joints as untreated controls. Articular cartilage samples were obtained at necropsy 1, 4, and 8 weeks after the last injection. Compared with controls, cartilage from injected joints had a loss of hematoxylin basophilia and decreased intensity of staining in safranin O fast green dye. Chondrocyte necrosis and hypocellularity were observed in all samples of cartilage from treated joints. Proteoglycan content and its rate of synthesis were reduced. There was a progressive loss of proteoglycan content, whereas proteoglycan synthesis increased somewhat 4 and 8 weeks after treatment. Collagen content was unchanged, but its rate of synthesis was markedly inhibited. Collagen synthesis did not recover, but remained decreased at 5 to 15% of the values from untreated cartilage. Water percentage was increased, but fibronectin content was not significantly different. A single injection of methylprednisolone acetate was also given into the right metacarpophalangeal joints of 3 of the 8 horses in this group, with the left joints serving as untreated controls. Sixteen weeks after the treatment, cartilage of the treated joints had a loss of histochemical staining and proteoglycan content was reduced to 50% of control values. The mean rate of proteoglycan synthesis and mean fibronectin content were increased, but the differences were not statistically significant (P greater than 0.05). Other variables were essentially unchanged.(ABSTRACT TRUNCATED AT 250 WORDS)