Effect of maternal polyunsaturated fatty acid concentration on transport by the human placenta
Effect of maternal polyunsaturated fatty acid concentration on transport by the human placenta
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DOI：
10.1159/000014115
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发表时间：
1999-06-01
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影响因子：
--
通讯作者：
Page, K
中科院分区：
文献类型：
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作者：
Haggarty, P;Ashton, J;Page, K
在正常妊娠的足月胎盘（n = 5）中研究了胎盘在控制胎儿脂肪酸供应中的作用。用含有脂肪酸生理混合物的改良克雷布斯林格氏溶液离体灌注母体侧90分钟，该溶液旨在模拟妊娠最后三个月测量的非酯化脂肪酸（NEFA）的组成（n = 10）-以及脂肪酸与人白蛋白的比例。α-亚麻酸（α LN）转移到胎儿循环的选择性与使用甘油三酯（TG）组合物时观察到的选择性（1.21 +/-0.04）没有显著差异，但对于AA（1.43 +/-0.12; p < 0.001）和二十二碳六烯酸（DHA; 2.02 +/-0.09; p = 0.048）有显著差异。转移的绝对速率（nmol.ml（-1））与使用TG母体灌注液组合物相比，对于LA，（0.562 +/- 0.038; p 0.50），α LN（0.130 +/- 0.009; p < 0.001）、花生四烯酸（AA; 0.218 +/- 0.022; p = 0.001）和DHA（0.383 +/- 0.04; p < 0.001）。因此，胎盘对aLN和DHA的选择性似乎对母体循环中脂肪酸混合物的变化相对不敏感，但对AA的选择性随着母体AA浓度的增加而增加。母体循环中DHA浓度增加8倍，则DHA向胎儿循环的转移增加13倍。对于AA浓度的2倍增加，转移增加8倍。对于α LN浓度增加1.3倍，转移增加2.1倍。这些结果表明，母体的浓度的个别脂肪酸，因此组成的母体饮食，可以有很大的影响多不饱和/长链多不饱和脂肪酸交付给胎儿。
The role of the placenta in controlling the supply of fatty acids to the fetus was investigated in term placentas (n = 5) from normal pregnancies. The maternal side was perfused ex vivo for 90 min with a modified Krebs Ringer solution containing a physiological mixture of fatty acids - designed to mimic the composition of non-esterified fatty acids (NEFA) measured in the last trimester of pregnancy (n = 10) - and ratio of fatty acid to human albumin. The selectivity for cr-linolenic acid (alpha LN) transfer to the fetal circulation was not significantly different from that observed when using the triglyceride (TG) composition (1.21 +/- 0.04), but significantly different for AA (1.43 +/- 0.12; p < 0.001) and docosahexaenoic acid (DHA; 2.02 +/- 0.09; p = 0.048). The absolute rate of transfer (nmol.ml(-1)) compared to that using the TG maternal perfusate composition was significantly different for LA (0.562 +/- 0.038; p 0.50), alpha LN (0.130 +/- 0.009; p < 0.001), arachidonic acid (AA; 0.218 +/- 0.022; p = 0.001) and DHA (0.383 +/- 0.04; p < 0.001). Thus, placental selectivity for aLN and DHA appears to be relatively unresponsive to changes in the mixture of fatty acids in the maternal circulation but the selectivity for AA increased with the increase in the maternal AA concentration. For an 8-fold increase in the concentration of DHA in the maternal circulation there was a 13-fold increase in the transfer of DHA to the fetal circulation. For a 2-fold increase in the concentration of AA, transfer was increased 8-fold. For a 1.3-fold increase in the concentration of alpha LN, transfer was increased 2.1-fold. These results suggest that the maternal concentration of individual fatty acids, and hence the composition of the maternal diet, can have large effects on polyunsaturated/long-chain polyunsaturated fatty acids delivery to the fetus.