Involvement of galanin receptor 2 and CaMKII in galanin-induced antinociception in periaqueductal grey of rats
Involvement of galanin receptor 2 and CaMKII in galanin-induced antinociception in periaqueductal grey of rats
复制标题
甘丙肽受体2和CaMKII参与甘丙肽诱导的大鼠导水管周围灰质镇痛作用
DOI：
10.1016/j.neulet.2015.08.005
复制
发表时间：
2015-09-14
影响因子：
2.5
通讯作者：
Yu, Long-Chuan
中科院分区：
文献类型：
--
作者：
Zhang, Xi-Yue;Zhang, Yi-Ming;Yu, Long-Chuan
本研究旨在探讨甘丙肽受体2（GalR 2）拮抗剂M871对甘丙肽诱导的中脑导水管周围灰质（PAG）抗伤害性感受的影响，以及Ca（2+）/钙调蛋白依赖性激酶II（CaMK II）在甘丙肽诱导的抗伤害性感受中的作用。PAG内注射甘丙肽诱导HWL对伤害性热和机械刺激的显著增加。PAG内注射GalR 2拮抗剂M871后，热刺激和机械刺激引起的HWL增加显著降低，表明GalR 2参与了甘丙肽诱导的大鼠PAG抗伤害感受作用。此外，大鼠接受PAG内注射甘丙肽，5分钟后PAG内给予CaMKII抑制剂MAP。PAG内注射MAP后，Galanin诱导的热刺激和机械刺激引起的HWL增加显著减少，提示CaMK Ⅱ参与了Galanin诱导的PAG抗伤害感受作用，阻断CaMK Ⅱ活性可抑制Galanin诱导的PAG抗伤害感受作用。我们的研究结果表明，GalR 2介导了甘丙肽诱导的大鼠PAG的抗伤害感受作用，CaMK Ⅱ可能参与了甘丙肽诱导的PAG的抗伤害感受作用。(C)2015爱思唯尔爱尔兰有限公司版权所有。
The present study was performed to explore the effect of the galanin receptor 2 (GalR2) antagonist M871 on the galanin-induced antinociception in periaqueductal grey (PAG), and an involvement of Ca(2+)/calmodulin-dependent kinase II (CaMKII) in the galanin-induced antinociception. Intra-PAG injection of galanin induced marked increases in HWLs to noxious thermal and mechanical stimulation. The increased HWLs to thermal and mechanical stimulation decreased significantly after intra-PAG administration of the GalR2 antagonist M871, indicating an involvement of GalR2 in the galanin-induced antinociception in PAG of rats. Furthermore, rats received intra-PAG injection of galanin, followed 5 min later by intra-PAG administration of the CaMKII inhibitor MAP. The galanin-induced increases in HWLs to thermal and mechanical stimulation decreased significantly after intra-PAG administration of MAP, indicating that there is an involvement of CaMKII in the galanin-induced antinociception in PAG, blockade the activity of CaMKII by MAP inhibits the galanin-induced antinociception in PAG of rats. Our results strongly indicate that the galanin-induced antinociception is mediated by GalR2 in the PAG, and CaMKII may be involved in the galanin-induced antinociception in PAG of rats. (C) 2015 Elsevier Ireland Ltd. All rights reserved.