Cloning and characterization of an Mx gene and its corresponding promoter from the zebrafish, Danio rerio
Cloning and characterization of an Mx gene and its corresponding promoter from the zebrafish, Danio rerio
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DOI：
10.1016/j.dci.2003.09.001
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发表时间：
2004-04-01
影响因子：
2.9
通讯作者：
Kim, CH
中科院分区：
文献类型：
--
作者：
Altmann, SM;Mellon, MT;Kim, CH
I型干扰素(IFN)是针对病毒的先天免疫反应的重要组成部分。干扰素的一个重要下游效应器是Mx基因，它只通过这一途径被激活。MX蛋白具有三个GTP结合域、动力蛋白家族特征和亮氨酸拉链基序。Mx基因在位于Mx启动子区域的干扰素刺激的反应元件(ISRE)被激活时被转录。本文介绍了斑马鱼(Danio Rerio)Mx基因及其相应启动子的克隆和分析。斑马鱼Mx的推导氨基酸序列包含与Mx蛋白相同的保守的GTP结合域、动力蛋白家族特征和亮氨酸拉链基序，与人类MxA的同源性为50%，与虹鲑鱼和大西洋鲑鱼的同源性为69%。斑马鱼肝细胞在已知的干扰素诱导剂多肌苷多胞苷(Poly[I：C])诱导下产生高水平的Mx mRNA。斑马鱼Mx启动子含有两个与许多干扰素诱导基因启动子区域同源的IRES，并且在纯化的斑马鱼干扰素或Poly诱导下能够驱动荧光素酶报告基因的转录[J：C]。(C)2003爱思唯尔有限公司。保留所有权利。
Type I interferons (IFNs) represent a crucial component of the innate immune response to viruses. An important downstream effector of IFN is the Mx gene, which is activated solely through this pathway. Mx proteins are characterized by a tripartite GTP-binding domain, dynamin family signature, and leucine zipper motif. Mx genes are transcribed upon activation of an interferon-stimulated response element (ISRE) located in the Mx promoter region. In this article, we describe the cloning and analysis of an Mx gene and its corresponding promoter from the zebrafish (Danio rerio). The deduced amino acid sequence of zebrafish Mx contains the conserved GTP-binding domain, dynamin family signature, and leucine zipper motif common to Mx proteins, and shows a 50% identity to human MxA and 69% identity both to rainbow trout and to Atlantic salmon. Zebrafish liver cells produced high levels of Mx mRNA in response to induction by the known IFN-inducer polyinosinic-polycytidylic acid (Poly[I:C]). The zebrafish Mx promoter contains two ISREs homologous to those found in the promoter regions of many IFN-inducible genes, and was able to drive transcription of a luciferase reporter gene when induced by either purified zebrafish IFN or Poly[J:C]. (C) 2003 Elsevier Ltd. All rights reserved.