Serological analysis of antigenic determinants on the env gene products of AKR dualtropic (MCF) murine leukemia viruses.
Serological analysis of antigenic determinants on the env gene products of AKR dualtropic (MCF) murine leukemia viruses.
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AKR 双向 (MCF) 鼠白血病病毒 env 基因产物的抗原决定簇的血清学分析。
DOI：
10.1016/0042-6822(80)90274-3
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发表时间：
1980
期刊：
影响因子：
3.7
通讯作者：
Nowinski,RC
中科院分区：
文献类型：
--
作者：
O'Donnell,PV;Nowinski,RC
一组八种单克隆抗体可识别鼠白血病病毒 (MuLV) 包膜蛋白（gp70 和 p15(E)）上的单个抗原决定簇（表位），已用于探测重组双向性 (MCF) 病毒的血清学特性。由于这些单克隆抗体中的六种显示出对内源性 N-共嗜性 MuLV 独有的表位的特异性，因此推测它们可能提供一种方法来确定双嗜性分离株的 env 基因中共嗜性信息的相对遗传贡献。预计这些病毒中亲嗜性特异性序列的丧失将反映为与亲嗜性特异性单克隆抗体的反应性丧失。这种预期被证明是正确的，因为与 AKR 小鼠的内源性亲嗜性 MuLV 反应的几种单克隆抗体未能与来自同一菌株的重组双嗜性 MuLV 分离株发生反应。此外，值得注意的是，单克隆抗体与不同双向病毒的“不反应性”以特征模式发生。这些模式的一个共同特征是来自所有 AKR 双向 MuLV 的 gp70a、gp70d 和 gp70 表位表达的坐标丢失。与亲嗜性特异性表位的常见缺失相反，gp70b、gp70c 和 p15(E) 表位在不同的重组病毒中被不同程度地缺失。
A panel of eight monoclonal antibodies recognizing individual antigenic determinan (epitopes) on the envelope proteins (gp70 and p15(E)) of murine leukemia virus (MuLV) has been used to probe the serological properties of recombinant dualtropic (MCF) viruses. Since six of these monoclonal antibodies showed specificity for epitopes that were exclusive to endogenous N-ecotropic MuLV, it was reasoned that they might provide a means to determine the relative genetic contribution of ecotropic information in theenvgene of dualtropic isolates. It was anticipated that the loss of ecotropic-specific sequences in these viruses would be reflected by a loss of reactivity with the ecotropic-specific monoclonal antibodies. This expectation proved to be correct, as several of the monoclonal antibodies that reacted with endogenous ecotropic MuLV of AKR mice failed to react with isolates of recombinant dualtropic MuLV from the same strain. In addition, it was noted that the “nonreactivity” of the monoclonal antibodies with different dualtropic viruses occurred in characteristic patterns. A common feature of the patterns was coordinate loss of expression of the gp70a, gp70d, and gp70eepitopes from all AKR dualtropic MuLV. In contrast to the common deletion of ecotropic-specific epitopes, the gp70b, gp70c, and p15(E)aepitopes were variably deleted in different recombinant viruses.