Increased proliferation and adhesion properties of human dental pulp stem cells in PLGA scaffolds via simulated microgravity
Increased proliferation and adhesion properties of human dental pulp stem cells in PLGA scaffolds via simulated microgravity
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DOI：
10.1111/iej.12441
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发表时间：
2016-02-01
影响因子：
5
通讯作者：
Niu, Y.
中科院分区：
文献类型：
--
作者：
He, L.;Pan, S.;Niu, Y.
目的探讨利用旋转细胞培养系统（RCCS）模拟微重力环境的可能性，并研究其对细胞增殖、粘附、人牙髓干细胞在聚乙烯上的迁移和细胞骨架的构建方法将在PLGA支架上生长的分离和鉴定的hDPSC暴露于模拟微重力（SMG）或正常重力（NG）。条件3天。采用MTT法、BrdU掺入法、流式细胞术和Western blotting检测SMG对hDPSCs增殖的影响。此外，进行免疫荧光检测、SEM观察以及细胞迁移和粘附测定，以比较经受SMG条件的hDPCSs中的粘附、迁移和细胞骨架变化。为了进一步研究机制，进行了人路径聚焦矩阵和粘附PCR阵列分析。学生的t检验被用于statistical analysis.ResultsSMG促进增殖和粘附，减少迁移和重组的hDPSCs的细胞骨架组织相比，NG组。PCR阵列分析显示SMG处理后，ITGA 6（整合素α-6）、ITGAV（整合素α-V）、ITGB 1（整合素β-1）、LAMB 1（层粘连蛋白β-1）和TNC（腱生蛋白-C）显著上调（P
AimTo explore the possibility of utilizing a rotary cell culture system (RCCS) to model simulated microgravity and investigate its effects on the proliferation, adhesion, migration and cytoskeletal organization of human dental pulp stem cells (hDPSCs) on poly (lactic-co-glycolic acid) (PLGA) scaffolds.MethodologyIsolated and identified hDPSCs grown on PLGA scaffolds were exposed to simulated microgravity (SMG) or normal gravity (NG) conditions for 3days. MTT cell proliferation assays, BrdU incorporation assays, flow cytometry analysis and Western blotting were undertaken to identify the proliferation ability of hDPSCs under SMG conditions. Additionally, immunofluorescence detection, SEM observations and cell migration and adhesion assays were performed to compare adhesion, migration and cytoskeletal changes in hDPCSs subjected to SMG conditions. To further investigate the mechanisms, human pathway-focused matrix and adhesion PCR array analyses were performed. The Student's t-test was used for statistical analyses.ResultsSMG promoted proliferation and adhesion, decreased migration and reorganized the cytoskeletal organization of hDPSCs compared with the NG group. PCR array analyses revealed that following SMG treatment, ITGA6 (integrin alpha-6), ITGAV (integrin alpha-V), ITGB1 (integrin beta-1), LAMB1 (laminin beta-1) and TNC (tenascin-C) were significantly upregulated (P