Total and high molecular weight but not trimeric or hexameric forms of adiponectin correlate with markers of the metabolic syndrome and liver injury in Thai subjects
Total and high molecular weight but not trimeric or hexameric forms of adiponectin correlate with markers of the metabolic syndrome and liver injury in Thai subjects
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DOI：
10.1210/jc.2007-0890
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发表时间：
2007-11-01
影响因子：
5.8
通讯作者：
Sweeney, Gary
中科院分区：
文献类型：
--
作者：
Liu, Ying;Retnakaran, Ravi;Sweeney, Gary
背景/目标：总脂联素减少与代谢紊乱有关，包括肥胖、糖尿病、脂肪肝和代谢综合征。虽然循环脂联素由三聚体、六聚体和高分子量（HMW）多聚体组成，但对这些异构体在人体中的特定代谢相关性的研究有限。因此，我们的目的是评估这些脂联素亚型与代谢和人体测量parameters.Design/参与者/设置：共53糖尿病和68 nondiabetes门诊患者进行了横断面代谢表征的协会。使用基于选择性蛋白酶介导消化的多聚体脂联素ELISA测量HMW、六聚体和三聚体脂联素的循环水平。在斯皮尔曼单变量分析中，总脂联素和高分子量脂联素水平与体重指数、空腹血糖、评估胰岛素抵抗的稳态模型、甘油三酯和丙氨酸氨基转移酶（ALT）（所有竖线r竖线>= 0.22; P < 0.05），HMW异构体与高密度脂蛋白胆固醇也呈正相关（r = 0.19; P = 0.036）。相比之下，六聚体和三聚体脂联素分别仅与体重指数（r = -0.23; P = 0.0102）和中上臂围（r = 0.21; P = 0.039）显著相关。在单独的正向逐步多元线性回归分析中，空腹血糖和ALT作为总脂联素和高分子量脂联素的独立负协变量出现，而六聚体和三聚体脂联素未发现独立协变量。此外，调整后的年龄，性别和糖尿病，平均ALT是最高的受试者在最低的三分之一的HMW脂联素，其次依次由中间和最高的三分之一，分别（趋势P = 0.028）。结论：HMW脂联素，但不是六聚体或三聚体，跟踪与总脂联素的代谢相关。此外，ALT和HMW脂联素之间存在独立的负相关。
Context/Objective: Decreased total adiponectin has been associated with metabolic disorders, including obesity, diabetes, fatty liver, and the metabolic syndrome. Although circulating adiponectin is composed of trimers, hexamers, and high molecular weight ( HMW) multimers, there has been limited study of the specific metabolic correlates of these isoforms in humans. Thus, our objective was to evaluate the associations of these adiponectin isoforms with metabolic and anthropometric parameters.Design/Participants/Setting: A total of 53 diabetic and 68 nondiabetic subjects attending outpatient clinics underwent cross-sectional metabolic characterization. Circulating levels of HMW, hexameric, and trimeric adiponectin were measured using a multimeric adiponectin ELISA based upon selective protease-mediated digestion.Results: On Spearman univariate analysis, both total and HMW adiponectin levels were inversely associated with body mass index, fasting glucose, homeostasis model of assessment of insulin resistance, triglycerides, and alanine aminotransferase ( ALT) ( all vertical bar r vertical bar >= 0.22; P < 0.05), with the HMW isoform also positively correlated with high-density lipoprotein cholesterol ( r = 0.19; P = 0.036). In contrast, hexameric and trimeric adiponectin were significantly associated with only body mass index ( r = -0.23; P = 0.0102) and mid-upper arm circumference ( r = 0.21; P = 0.039), respectively. On separate forward stepwise multiple linear regression analyses, fasting glucose and ALT emerged as independent, negative covariates of both total and HMW adiponectin, whereas no independent covariates of hexameric and trimeric adiponectin were identified. Furthermore, after adjustment for age, gender, and diabetes, mean ALT was highest in subjects in the lowest tertile of HMW adiponectin, followed in turn by the middle and highest tertiles, respectively ( trend P = 0.028).Conclusions: HMW adiponectin, but not hexameric or trimeric, tracks with the metabolic correlates of total adiponectin. Furthermore, an independent inverse association exists between ALT and HMW adiponectin.