Skin test and tick immune status in susceptible and resistant cattle in Brazil
Skin test and tick immune status in susceptible and resistant cattle in Brazil
复制标题
DOI：
10.1111/j.1749-6632.2000.tb05338.x
复制
发表时间：
2000-01-01
期刊：
影响因子：
--
通讯作者：
Szabó, MPJ
中科院分区：
文献类型：
--
作者：
Bechara, GH;Morelli, J;Szabó, MPJ
使用皮肤过敏试验（CHT）来关联宿主对蜱的抵抗力和对欧洲和印度牛中未喂食的牛蜱微小牛蜱幼虫提取物 ULE 引起的反应类型。二十头小牛被分成四组，每组五只动物：未受感染的或感染前的印度或欧洲牛。通过在小牛耳中皮内接种 0.1 ml ULE 牛蜱 B. microplus（50 杯蛋白质）来诱导 CHT。在接种前和接种后 10 分钟、1、2、6、18、24、48、72、96 和 144 小时 (PI) 使用卡尺测量耳朵厚度。感染前的欧洲小牛仅表现出立即型反应，并在感染后 10 分钟时出现最大反应（耳朵厚度增加 75%）。另一方面，感染前的印度小牛在感染后 10 分钟至一小时之间表现出立即反应，最大反应（耳朵厚度增加 70%），并在感染后 72 小时出现延迟型反应（耳朵厚度增加 60%）。这些结果指出了牛的细胞免疫反应在表达对牛蜱 B. microplus 的抗性中的关键作用。皮肤测试可能有助于根据对蜱的敏感性/抵抗力对牛进行排名。
A cutaneous hypersensitivity test (CHT) was used to correlate host resistance to ticks and type of reaction elicited to unfed larval extract-ULE of the cattle tick Boophilus microplus in European and Indian cattle. Twenty calves were separated into four groups of five animals each: naive or preinfested Indian or European cattle. CHT was induced by intradermal inoculation of 0.1 ml of ULE cattle tick B. microplus (50 mug protein) in the calf ear. Ear thickness was measured using calipers before and 10 min, 1, 2, 6, 18, 24, 48, 72, 96, and 144 h postinoculation (PI). Preinfested European calves showed only an immediate type reaction with maximum response (75% increase in ear thickness) at 10 min PI. On the other hand, preinfested Indian calves presented an immediate response with maximum reaction (70% increase in ear thickness) between 10 min and one hour PI, and a delayed type reaction at 72 h PI (60% increase in ear thickness). These results point out the crucial role of the cellular immune response of cattle in the expression of resistance to cattle tick B. microplus. Skin test might be useful in the ranking of cattle according to the susceptibility/resistance to ticks.