Crystal structure of the PSPTO-PSP protein from Pseudomonas syringae pv. tomato str. DC3000 in complex with D-glucose.
Crystal structure of the PSPTO-PSP protein from Pseudomonas syringae pv. tomato str. DC3000 in complex with D-glucose.
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DOI：
10.1016/j.bbrc.2010.05.071
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发表时间：
2010-06
影响因子：
3.1
通讯作者：
Hongmei Zhang;Yu Gao;Mei Li;Wenrui Chang
中科院分区：
文献类型：
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作者：
Hongmei Zhang;Yu Gao;Mei Li;Wenrui Chang
高氯酸可溶性蛋白(PSP)是一种内切核酸酶，根据序列的相似性被归入YjgF/YER057c/UK114家族。这些家族成员无处不在，在进化上高度保守，参与调节基本的细胞代谢。在这里，我们展示了来自紫丁香假单胞菌的PSP蛋白的2.1？晶体结构。番茄串。DC3000(PSPTO-PSP)，与d-葡萄糖形成络合物。PSPTO-PSP的四级结构为同源三聚体。葡萄糖位于两个单体之间的空腔中。比较配体和YjgF/YER057c/UK114家族同系物之间的氢键，证实了PSPTO-PSP保守的Arg103是该空腔中与配体结合的关键氨基酸。这表明PSPTO-PSP参与细胞的基本机制是由占据该活性部位的葡萄糖调节的。
The perchloric acid-soluble protein (PSP) is an endoribonuclease and on the basis of sequence similarity has been assigned to the YjgF/YER057c/UK114 family. These family members are ubiquitous and highly conserved in evolution, and participate in regulating basic cellular metabolism. Here we present the 2.1Å crystal structure of the PSP protein from Pseudomonas syringae pv. tomato str. DC3000 (PSPTO-PSP), in complex with d-glucose. The quaternary structure of PSPTO-PSP is a homologous trimer. Glucose is located in the cavity between each two monomers. Comparison of the hydrogen bonds between ligands and YjgF/YER057c/UK114 family homologues confirms that the conserved Arg103of PSPTO-PSP is a key amino acid in this cavity for ligand binding. It indicated that the involvement of PSPTO-PSP in essential cellular mechanism was regulated by glucose occupying this active site.