The Anopheles gambiae complex:: a new species from ethiopia
The Anopheles gambiae complex:: a new species from ethiopia
复制标题
DOI：
10.1016/s0035-9203(98)90761-1
复制
发表时间：
1998-03-01
影响因子：
2.2
通讯作者：
Fettene, M
中科院分区：
文献类型：
--
作者：
Hunt, RH;Coetzee, M;Fettene, M
历史上，埃塞俄比亚冈比亚按蚊复合体的成员在染色体上被鉴定为A。arabiensis或A.四环带的最近在埃塞俄比亚吉玛地区（位于阿迪斯西南部）采集到了29个A.基于标准的聚合酶链反应（PCR）鉴定方法，鉴定了四环纹夜蛾。“野生”雌性被诱导产卵，后代作为个体家庭饲养。将所得成虫与实验室A.原产于南非克鲁格国家公园的四环龙。杂交后代仅从群体殖民地雌性与雄性杂交获得。这种杂交产生的雌性/雄性性别比为0.48。雄性后代不育，杂交雌性的卵巢多线染色体表现出典型的不联会，正如A内种间杂交所预期的那样。冈比亚情结。X染色体虽然具有明显的同源带型，但通常是完全不联会的。所有常染色体均为同源序列。在野生和杂交样品中缺乏倒位杂合子，可能只是样品量小的反映。在埃塞俄比亚种被正式描述和命名之前，由于其与该种非常相似，暂时将其命名为四环按蚊种B。
Historically, members of the Anopheles gambiae complex from Ethiopia have been identified chromosomally as either A. arabiensis or A. quadriannulatus. Recent collections from the Jimma area in Ethiopia, southwest of Addis Ababa, revealed 29 specimens of A. quadriannulatus based on the standard polymerase chain reaction (PCR) identification method. 'Wild' females were induced to lay eggs and the progeny reared as individual families. Resulting adults were cross-mated to a laboratory colony strain of A. quadriannulatus originating from the Kruger National Park, South Africa. Hybrid progeny were obtained only from the colony femalexEthiopian male cross. This cross produced a female/male sex ratio of 0.48. Male offspring were sterile and ovarian polytene chromosomes from hybrid females showed typical asynapsis as expected in interspecific crosses within the A. gambiae complex. The X chromosomes, although apparently having homosequential banding patterns, were usually totally asynapsed. All autosomes were homosequential. The lack of inversion heterozygotes, in both the wild and hybrid samples, may simply be a reflection of the small sample size. Until such time as the Ethiopian species can be formally described and assigned a scientific name, it is provisionally designated Anopheles quadriannulatus species B because of its close similarity to this species.