A cnidarian homologue of translationally controlled tumor protein (P23/TCTP).
A cnidarian homologue of translationally controlled tumor protein (P23/TCTP).
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翻译控制肿瘤蛋白 (P23/TCTP) 的刺胞动物同源物。
DOI：
10.1007/s004270000088
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发表时间：
2000
影响因子：
2.4
通讯作者：
SarrasJr,MP
中科院分区：
文献类型：
--
作者：
Yan,L;Fei,K;Bridge,D;SarrasJr,MP
在最古老的后生动物中克隆了一个与P23同源的蛋白质，即翻译控制的肿瘤蛋白(TCTP)，它是迄今为止发现的最古老的后生动物。这种无脊椎动物的P23/TCTP与其他无脊椎动物和脊椎动物物种相比保存得很好。用鼠重组P23/TCTP多克隆抗体对Hydracell裂解产物进行免疫印迹检测，证实了HydraP23/TCTP的表达。分别用原位杂交和免疫组织化学方法研究了HydraP23/TCTP基因在体轴上的空间分布，HydraP23/TCTP在转录和翻译水平上均受调控。在体柱中的细胞亚群中检测到高水平的P23/TCTPmRNA。相反，在头部和足部的分化细胞中没有明显的mRNA表达。巧合的是，P23/TCTP蛋白也集中在体柱，头部和足部没有检测到蛋白质。然而，尽管在体柱的外胚层和内胚层中都存在P23/TCTPmRNA，但其蛋白定位于内胚层细胞，这表明在翻译水平上存在一种调控机制。综上所述，P23/TCTP在Hydra中的表达模式与细胞增殖活跃的区域相关，其表达被排除在发生末端分化的区域之外。
A protein homologous to P23, or translationally controlled tumor protein (TCTP), was cloned inHydra vulgaris, the most ancient type of metazoan from which P23/TCTP has been characterized to date.HydraP23/TCTP is composed of 184 amino acids and is encoded by a single mRNA of 700 bp. This invertebrate P23/TCTP is well conserved compared to those of other invertebrate and vertebrate species. Expression ofHydraP23/TCTP was confirmed by western blot ofHydracell lysates using a polyclonal antibody against murine recombinant P23/TCTP. Spatial distribution of P23/TCTP mRNA and protein inHydrawas studied using in situ hybridization and immunostaining, respectively.HydraP23/TCTP expression along the longitudinal body axis is regulated at both the transcriptional and the translational level. High levels ofP23/TCTPmRNA were detected in a subpopulation of cells in the body column. In contrast, no mRNA was evident in the differentiated cells of the head and the foot regions. Coincidentally, P23/TCTP protein also concentrates to the body column, with no detectable protein in the head and foot region. However, despite the existence ofP23/TCTPmRNA in both the ectoderm and endoderm in the body column, its protein is localized to the endodermal cells, suggesting a regulatory mechanism at the translational level. Taken together, the expression pattern of P23/TCTP inHydracorrelates with regions in which cell proliferation is actively occurring and its expression is excluded from regions where terminal differentiation has occurred.