Quisqualate and ACPD are agonists for a glutamate-activated current in identified Aplysia neurons.
Quisqualate and ACPD are agonists for a glutamate-activated current in identified Aplysia neurons.
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Quisqualate 和 ACPD 是已鉴定的海兔神经元中谷氨酸激活电流的激动剂。
DOI：
10.1152/jn.1993.69.1.143
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发表时间：
1993
影响因子：
2.5
通讯作者：
Levitan,IB
中科院分区：
文献类型：
--
作者：
Katz,PS;Levitan,IB
1.谷氨酸是脊椎动物和无脊椎动物中重要的神经递质，但软体动物谷氨酸受体的特性及其与脊椎动物受体的关系尚不完整。本研究使用双电极电压钳来表征谷氨酸诱发电流的培养神经元从海洋腹足类软体动物加州滨对虾。2.所确定的颊神经节神经元，B1和B2，显示两个截然不同的电流响应的压力施加谷氨酸。一种反应是类似于在其他大的颊神经节神经元中发现的脱敏氯电流。另一种是强烈向外整流的非脱敏钾电流。3.钾电流响应对谷氨酸的敏感性高于氯离子响应。4.在原代细胞培养中分离的单个神经元表现出两种电流的不同相对比例。5.谷氨酸激动剂使君子酸和（1 S，3R）-氨基环戊烷-1，3-二羧酸特异性地引起钾反应，但不引起氯反应。谷氨酸激动剂（RS）-α-氨基-3-羟基-5-甲基-4-异恶唑丙酸未引起任何明显反应。红藻氨酸和N-甲基-D-天冬氨酸对这些细胞也没有影响。另一种谷氨酸类似物鹅膏蕈氨酸引起瞬时氯电流，但不引起钾电流。6.这些结果表明，谷氨酸受体介导的外向钾反应不符合其药理学概况的任何已知的脊椎动物谷氨酸受体类型。
1. Glutamate is an important neurotransmitter in both vertebrates and invertebrates, yet the characterization of molluscan glutamate receptors and their relationships to vertebrate receptors is incomplete. This study uses two-electrode voltage clamp to characterize glutamate-evoked currents in cultured neurons from the marine gastropod mollusk Aplysia californica. 2. The identified buccal ganglion neurons, B1 and B2, display two pharmacologically distinct current responses to pressure-applied glutamate. One response is a desensitizing chloride current similar to that found in other large buccal ganglion neurons. The other is a nondesensitizing potassium current that is strongly outwardly rectifying. 3. The potassium current response has a higher sensitivity to glutamate than the chloride response. 4. Individual neurons, isolated in primary cell culture, exhibit different relative proportions of the two currents. 5. The glutamate agonists quisqualate and (1S,3R)-aminocyclopentane-1,3-dicarboxylic acid specifically evoke the potassium response but not the chloride response. The glutamate agonist (RS)-alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid does not evoke any appreciable response. Kainate and N-methyl-D-aspartate also have no effect on these cells. Another glutamate analogue, ibotenate, evokes the transient chloride current but not the potassium current. 6. These results indicate that the glutamate receptor mediating the outward potassium response does not conform in its pharmacological profile to any of the known vertebrate glutamate receptor types.