Induction of diploid gynogenesis by heat shock treatment in silver barb (Barbonymus gonionotus)
Induction of diploid gynogenesis by heat shock treatment in silver barb (Barbonymus gonionotus)
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DOI：
10.1016/j.aquaculture.2019.02.015
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发表时间：
2019-04
期刊：
影响因子：
4.5
通讯作者：
M. R. Rahman;M. R. I. Sarder;A. A. Nishat-A.;R. Islam
中科院分区：
文献类型：
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作者：
M. R. Rahman;M. R. I. Sarder;A. A. Nishat-A.;R. Islam
本研究采用热激处理方法诱导银鱼（Barbonalgonionotus）的二倍体雌核发育，以获得单性全雌群体。当精子悬液浓度为8 × 108 ml-1时，在196μWcm-2的紫外光照射下照射1.5min，精子悬液中的精子浓度达到最大值。受精1.5 min后，在40 °C下热激1 min，雌核发育二倍化，产生40.36 ± 3.46%的雌核原细胞。同样，在受精27.5分钟后，使用相同的热休克处理实现了有丝分裂-雌核发育二倍体化，产生33.80 ± 2.84%的有丝分裂-雌核发育。雌核发育个体的鉴定采用染色体核型、性腺性别鉴定和DNA微卫星分析。染色体核型分析表明，单倍体有23（N）条染色体，而减数分裂和有丝分裂雌核发育二倍体和对照有46（2 N）条染色体。性别鉴定结果表明，雌核发育中期和有丝分裂中期几乎全为雌性，雌性比例分别为98.18%~ 100%和96.77%~ 100%。对照组的平均性别比为54.4：52.8，女性：男性接近1：1。DNA微卫星分析表明，雌核发育的幼鱼含有与其母亲相同的等位基因，而雌核发育的幼鱼通过固定两个母亲等位基因中的任何一个而成为纯合的。没有一个雌核生物有父系遗传。产生的二倍体雌核发育细胞和有丝分裂雌核发育细胞非常有价值，因为它们几乎都是雌性，并且由于它们更快的生长速度，预期产量更高。此外，利用雄性化激素对雌核发育幼虫和丝裂雌核发育幼虫进行性反转，将有助于产生性反转的雄性（XX），这些雄性（XX）能够在与遗传雌性（XX）杂交时产生单性全雌性群体。
The research work was carried out for induction of diploid meyo- and mito- gynogenesis in silver barb(Barbonymus gonionotus)through heat shock treatment in order to produce monosex all-female population. Optimal UV-irradiation achieved when the sperm suspension containing 8 × 108ml−1was exposed to a UV dose of 196μWcm−2for 1.5 min. Diploidization of gynogenetics was done by heat shock at 40 °C for 1 min after 1.5 min of fertilization that produced 40.36 ± 3.46% meyo-gynogens. Similarly, mito-gynogenetic diploidization was achieved using the same heat shock treatment after 27.5 min of fertilization that yielded 33.80 ± 2.84% mito-gynogens. The gynogens were identified by karyotype, gonad identification through sexing and DNA microsatellite analysis. The karyotype analysis showed that the haploids have 23 (N) chromosomes, and the meyo- and mito-gynogenetic diploids and controls have 46 (2 N) chromosomes. Sexing of fish demonstrated that the meyo- and mito-gynogens were nearly all-female, ranging 98.18 to 100% and 96.77 to 100% female sex respectively. The control group had a mean sex ratio of 54.4:52.8, nearly 1:1 female: male sex. DNA microsatellite analysis revealed that the meyo-gynogenetic fry contained the alleles same as their mother and the mito-gynogenetic fry became homozygous by fixing any of the two maternal alleles. None of the gynogens had paternal inheritance. The diploid meyo- and mito-gynogens produced are much valuable as they are nearly all-female and a higher production is expected due to their faster growth rate. In addition, sex inversion of meyo- and mito-gynogenetic larvae using masculinizing hormone will facilitate to produce sex-reversed males (XX) those are capable to generate monosex all-female population upon crossing with genetic females (XX).