Histological features of the degenerating intervertebral disc in a goat disc-injury model.
Histological features of the degenerating intervertebral disc in a goat disc-injury model.
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DOI：
10.1097/brs.0b013e3181f60b39
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发表时间：
2011-09-01
期刊：
影响因子：
3
通讯作者：
Anderson DG
中科院分区：
文献类型：
--
作者：
Zhang Y;Drapeau S;An HS;Markova D;Lenart BA;Anderson DG
建立山羊椎间盘退变大动物模型的体内研究。确定诱导山羊IVD变性的最佳方法，适用于测试椎间盘再生疗法。尽管啮齿动物、兔和其他小动物研究是有用的，但这些物种中IVD的狭窄尺寸限制了需要注射相关体积的治疗剂或植入工程化组织构建体的研究。在本研究中，选择山羊是因为它们的IVD的大小和形状与成年人类相当。使用了一种微创方法，未对成年山羊（n = 6）造成显著的发病率或死亡率。在X线透视引导下，用4.5 mm钻头或#15或#10手术刀片损伤山羊腰椎IVD。损伤后两个月，对山羊实施安乐死，分离其IVD与相邻终板，脱钙并染色。根据增田等人先前描述的兔IVD的组织学特征，Hoogendoorn等人描述的山羊IVD，和Boos等人描述的人IVD。评估了我们评分系统的评分者间一致性（加权Kappa值= 0.6646）。Mann-Whitney检验用于比较损伤的IVD与未损伤的对照。4.5 mm钻头插入15 mm深度导致组织学评分显著高于未损伤对照组（p = 0.01）。与未受伤的对照组相比，使用#15或#10刀片造成的损伤未导致组织学评分增加。对各种损伤的比较表明，使用4.5 mm钻头导致最显著的组织学变化。
An in vivo study to develop a goat large-animal model for intervertebral disc (IVD) degeneration. To determine an optimal method for inducing goat IVD degeneration suitable for testing disc regeneration therapies. Although rodent, rabbit, and other small animal studies are useful, the narrow dimensions of IVDs in these species limit studies requiring injection of a relevant volume of therapeutics or implantation of engineered tissue constructs. For this study, the goat was selected because the size and shape of their IVDs are comparable to those of adult humans. A minimally invasive approach that did not cause significant morbidity or mortality to adult goats (n = 6) was used. Under fluoroscopic guidance, goat lumbar IVDs were injured with a 4.5 mm drill bit or #15 or #10 surgical blades. Two months post-injury, the goats were euthanized and their IVDs with adjacent endplates were isolated, decalcified and stained. A numerical histological scale to categorize the degree of goat IVD degeneration was developed based on the histological features of rabbit IVDs previously described by Masuda et al., goat IVDs described by Hoogendoorn et al., and human IVDs described by Boos et al. The inter-rater agreement of our scoring system was assessed (weighted Kappa value = 0.6646). Mann-Whitney tests were used to compare the injured IVDs with uninjured control. A 4.5 mm drill bit inserted to a 15 mm depth resulted in a significantly higher histological score compared to uninjured controls (p = 0.01). Injury with a #15 or #10 blade did not result in increased histological scores compared with uninjured controls. A comparison of the various injuries inflicted showed that the use of a 4.5 mm drill bit resulted in the most significant histological changes.