Leukemia inhibitory factor induces multi-lineage differentiation of adult stem-like cells in kidney via kidney-specific cadherin 16
Leukemia inhibitory factor induces multi-lineage differentiation of adult stem-like cells in kidney via kidney-specific cadherin 16
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DOI：
10.1016/j.bbrc.2004.12.167
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发表时间：
2005-03-04
影响因子：
3.1
通讯作者：
Fujita, T
中科院分区：
文献类型：
--
作者：
Hishikawa, K;Marumo, T;Fujita, T
据报道，侧群（SP）是富含干细胞的群。在白血病抑制因子 (LIF) 存在的情况下，培养的肾 SP 细胞在胶原凝胶中分化为多谱系，但在不含细胞粘附因子的合成聚合物中则不会分化。在培养的肾 SP 细胞中，肾特异性钙粘蛋白 16 的基因表达在胶原凝胶中特异性上调，但在合成聚合物中则没有。此外，使用 siRNA 降低钙粘蛋白 16 的表达消除了胶原凝胶中 LIF 诱导的肾 SP 的多谱系分化。这些结果表明，LIF 通过钙粘蛋白 16 诱导肾脏中成体干细胞样细胞的多谱系分化。(C) 2005 Elsevier Inc. 保留所有权利。
Side population (SP) is reported to be a stem cell-rich population. In the presence of leukemia inhibitory factor (LIF), cultured kidney SP cells differentiated into multi-lineage in collagen gel but not in synthesized polymer that has no cell adhesion factor. In cultured kidney SP cells, gene expression of kidney-specific cadherin 16 was specifically upregulated in collagen gel but not in synthesized polymer. Moreover, decreasing cadherin 16 expression using siRNA abolished LIF-induced multi-lineage differentiation of kidney SP in collagen gel. These results indicated that LIF induced multi-lineage differentiation of adult stem-like cells in kidney via cadherin 16. (C) 2005 Elsevier Inc. All rights reserved.