PCR detection of host and HIV-1 sequences from archival brain tissue.
PCR detection of host and HIV-1 sequences from archival brain tissue.
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DOI：
10.3109/13550280009013160
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发表时间：
2000
影响因子：
3.2
通讯作者：
Karen Müller Smith;Keith A. Crandall;Michelle L. Kneissl;Bradford A. Navia
中科院分区：
文献类型：
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作者：
Karen Müller Smith;Keith A. Crandall;Michelle L. Kneissl;Bradford A. Navia
CCR5和CCR2b的突变最近被证明影响艾滋病的疾病进展。这些宿主基因型在艾滋病痴呆复合体（ADC）中的作用也被假设，但仍不清楚。此外，来自HIV-1的脑源性包膜序列与ADC有关，但它们对发病机制的具体贡献仍不确定。本研究成功地利用PCR技术从ADC患者和非ADC患者的石蜡包埋组织中分离出宿主CCR5和CCR2b以及HIV-1 V3序列。来自档案组织的PCR扩增为研究宿主中潜在的神经保护因子与HIV毒力决定因素之间的相互作用提供了一种新的方法，这种相互作用可能导致对ADC的易感性差异。
Mutations in CCR5 and CCR2b have been recently shown to affect disease progression towards AIDS. A role for these host genotypes in AIDS dementia complex (ADC) has also been postulated but remains unclear. Additionally, brain-derived envelope sequences from HIV-1 have been associated with ADC but their specific contribution to pathogenesis remains uncertain. This study demonstrates the successful use of PCR techniques to isolate host CCR5 and CCR2b, and HIV-1 V3 sequences from paraffin embedded tissues from patients with and without ADC. PCR amplification from archival tissue offers a novel approach for studying the interactions between potential neuroprotective elements in the host and virulence determinants in HIV that may contribute to differences in susceptibility to ADC.