Translocation of an outer membrane protein into prey cytoplasmic membranes by bdellovibrios.
Translocation of an outer membrane protein into prey cytoplasmic membranes by bdellovibrios.
复制标题
蛭弧菌将外膜蛋白易位到猎物细胞质膜中。
DOI：
10.1128/jb.176.4.948-952.1994
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发表时间：
1994
影响因子：
3.2
通讯作者：
Karp,MA
中科院分区：
文献类型：
--
作者：
Tudor,JJ;Karp,MA
噬菌弧菌攻击大肠杆菌等猎物细胞后几分钟内，猎物的细胞质膜就会发生改变。纯化的侵入猎物细胞（bdelloplast）膜的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳揭示了非细胞质膜蛋白的出现。在未侵入的大肠杆菌膜的制备物中未观察到该蛋白质，并且其迁移方式与大肠杆菌 OmpF 相似。蛭形体细胞质膜制剂的等电聚焦和二维凝胶电泳也揭示了具有与 OmpF 和主要蛭弧菌外膜蛋白相似的电泳特性的蛋白质的存在。该蛋白质在攻击后几分钟内出现在细胞质膜制剂中，并在大部分周质内发育周期中持续存在。这种蛋白质的出现与我们的假设一致，即蛭弧菌将孔蛋白易位到蛭体细胞质膜中以杀死猎物并获得细胞质内容物进行生长。
Within minutes of Bdellovibrio bacteriovorus attack on prey cells, such as Escherichia coli, the cytoplasmic membrane of the prey is altered. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of purified invaded prey cell (bdelloplast) membranes revealed the appearance of a noncytoplasmic membrane protein. This protein is not observed in preparations of noninvaded E. coli membranes and migrates in a manner similar to that of E. coli OmpF. Isoelectric focusing and two-dimensional gel electrophoresis of bdelloplast cytoplasmic membrane preparations also revealed the presence of a protein with electrophoretic properties similar to those of OmpF and the major Bdellovibrio outer membrane proteins. The protein appears in cytoplasmic membrane preparations within minutes of attack and persists throughout most of the intraperiplasmic developmental cycle. The appearance of this protein is consistent with our hypothesis that bdellovibrios translocate a pore protein into the bdelloplast cytoplasmic membrane to kill their prey and to gain access to the cytoplasmic contents for growth.