Selective protein adsorption property and characterization of nano-crystalline zinc-containing hydroxyapatite
Selective protein adsorption property and characterization of nano-crystalline zinc-containing hydroxyapatite
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DOI：
10.1016/j.actbio.2005.09.002
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发表时间：
2006-01-01
影响因子：
9.7
通讯作者：
Babonneau, F
中科院分区：
文献类型：
--
作者：
Fujii, E;Ohkubo, M;Babonneau, F
以牛血清白蛋白（BSA）和致病蛋白β（2）-微球蛋白（β（2）-MG）为模型蛋白，采用湿化学法制备纳米晶含锌羟基磷灰石（ZnHAp），并考察其对必需蛋白的选择性吸附。透射电镜观察和X射线衍射分析表明，Zn含量的增加导致ZnHAps晶粒变小，并且ZnHAps的比表面积相应地随着Zn含量的增加而增加。此外，尽管比表面积增加，但随着Zn含量的增加，ZnHAp颗粒上BSA的吸附量减少。由此得出结论，纳米晶ZnHAps对β(2)-MG具有高选择性吸附特性。 (c) 2005 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
Nano-crystalline Zn-containing hydroxyapatite (ZnHAp) was prepared by the wet-chemical method and the selective adsorption of essential proteins was examined, taking bovine serum albumin (BSA) and pathogenic protein such as beta(2)-microglobulin (beta(2)-MG) as model proteins. Transmission electron microscopy observation and X-ray diffraction analysis indicated that the increase of Zn content led to smaller crystallites and their specific surface area of ZnHAps increased with increasing Zn content, accordingly. Furthermore, the amounts of BSA adsorption on ZnHAp particles decreased with increasing Zn content in spite of the increase in the specific surface area. It is thus concluded that nano-crystalline ZnHAps had a highly selective adsorption property with regard to beta(2)-MG. (c) 2005 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.