Denver Papillae Protocol for Objective Analysis of Fungiform Papillae.
Denver Papillae Protocol for Objective Analysis of Fungiform Papillae.
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DOI：
10.3791/52860
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发表时间：
2015-06-08
期刊：
影响因子：
--
通讯作者：
Santorico SA
中科院分区：
文献类型：
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作者：
Nuessle TM;Garneau NL;Sloan MM;Santorico SA
丹佛乳头协议的目标是使用二分键来定义菌状乳头 (FP) 的特征并确定其优先级，以确保评分者之间的评分一致。该协议是基于过去二十年使用 FP 作为味孔密度代理的味道研究中产生的需求而构建的。历史上，FP 密度是使用 Miller & Reedy 1990 年的形态特征进行分析的：圆形、染色较浅、较大和升高。在这项工作中，作者预先警告说，需要对 FP 形态学进行更严格的定义。尽管呼吁采取行动，但后续文献却很少，大多数研究仍然引用 Miller & Reedy 的原创作品。因此，FP 密度报告变化很大，再加上样本量较小，可能会导致关于 FP 在味觉敏感度中的作用的不同结论。味觉遗传学实验室探索了计数中这种明显的不一致，发现评分者对这些特征的重要性的优先顺序不同，并且对于乳头何时具有部分（但不是全部）报告的 FP 品质没有指导。这种主观性的结果是同一舌头图像的 FP 计数存在很大差异。丹佛乳头协议的开发是为了通过使用二分键来补救这一后果，该二分键进一步定义和优先考虑了 Miller & Reedy 提出的特征的重要性。所提出的方法可以帮助口味和营养研究领域的研究人员创建一种量化 FP 的标准方法。
The goal of the Denver Papillae Protocol is to use a dichotomous key to define and prioritize the characteristics of fungiform papillae (FP) to ensure consistent scoring between scorers. This protocol builds off of a need that has arisen from the last two decades of taste research using FP as a proxy for taste pore density. FP density has historically been analyzed using Miller & Reedy’s 1990 characterizations of their morphology: round, stained lighter, large, and elevated. In this work, the authors forewarned that stricter definitions of FP morphology needed to be outlined. Despite this call to action, follow up literature has been scarce, with most studies continuing to cite Miller & Reedy’s original work. Consequently, FP density reports have been highly variable and, combined with small sample sizes, may contribute to the discrepant conclusions on the role of FP in taste sensitivity. The Genetics of Taste Lab explored this apparent inconsistency in counting and found that scorers were individually prioritizing the importance of these characteristics differently and had no guidance for when a papilla had some, but not all, of the reported qualities of FP. The result of this subjectivity is highly variable FP counts of the same tongue image. The Denver Papillae Protocol has been developed to remedy this consequence through use of a dichotomous key that further defines and prioritizes the importance of the characteristics put forth by Miller & Reedy. The proposed method could help create a standard way to quantify FP for researchers in the field of taste and nutritional studies.