An efficient chemoenzymatic production of small molecule glucosides with in situ UDP‐glucose recycling
An efficient chemoenzymatic production of small molecule glucosides with in situ UDP‐glucose recycling
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DOI：
10.1016/j.febslet.2007.04.074
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发表时间：
2007-05
期刊：
影响因子：
3.5
通讯作者：
Sayaka Masada;Yoji Kawase;M. Nagatoshi;Y. Oguchi;Kazuyoshi Terasaka;H. Mizukami
中科院分区：
文献类型：
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作者：
Sayaka Masada;Yoji Kawase;M. Nagatoshi;Y. Oguchi;Kazuyoshi Terasaka;H. Mizukami
描述了一种有效酶促合成姜黄素糖苷的一锅系统。该方法耦合了两种重组酶 UDP-葡萄糖的活性：来自长春花的姜黄素葡萄糖基转移酶 (CaUGT2) 和来自拟南芥的蔗糖合酶 (AtSUS1)。 UDP 是 UDP-葡萄糖基转移酶的产物抑制剂，从系统中去除并通过第二种酶 AtSUS1 用于再生 UDP-葡萄糖。生产率提高了数倍，并且最初添加到反应混合物中的UDP-葡萄糖可以减少到正常水平的十分之一。通过将UDP-葡萄糖再生系统与葡萄糖基转移酶偶联来提高糖基化效率的概念应该适用于多种葡萄糖苷的酶促生产。
A one-pot system for efficient enzymatic synthesis of curcumin glucosides is described. The method couples the activities of two recombinant enzymes, UDP-glucose: curcumin glucosyltransferase from Catharanthus roseus (CaUGT2) and sucrose synthase from Arabidopsis thaliana (AtSUS1). UDP, a product inhibitor of UDP-glucosyltransferase, was removed from the system and used for regeneration of UDP-glucose by the second enzyme, AtSUS1. The productivity was increased several-fold and UDP-glucose initially added to the reaction mixture could be reduced to one-tenth of the normal level. The concept of enhancing glucosylation efficiency by coupling a UDP-glucose regeneration system with glucosyltransferases should be applicable to enzymatic production of a wide range of glucosides.