Regulatory mechanisms of ecdysone-inducible Blimp-1 encoding a transcriptional repressor that is important for the prepupal development in Drosophila
Regulatory mechanisms of ecdysone-inducible Blimp-1 encoding a transcriptional repressor that is important for the prepupal development in Drosophila
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DOI：
10.1111/j.1440-169x.2011.01276.x
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发表时间：
2011-06-01
影响因子：
2.5
通讯作者：
Ueda, Hitoshi
中科院分区：
文献类型：
--
作者：
Akagi, Kazutaka;Ueda, Hitoshi
Blimp-1 是一种蜕皮激素诱导型转录因子，在蛹期早期表达。其消失的时间决定了 FTZ-F1 基因的表达时间，其暂时受限的表达对于预蛹发育至关重要。为了阐明Blimp-1基因表达的终止机制，我们使用器官培养系统检查了Blimp-1基因的调控。结果表明，即使在长期暴露于 20-羟基蜕皮激素后，Blimp-1 基因在取自低蜕皮激素时期的培养器官中仍能转录，而众所周知的早期基因（如 E75A）在相同条件下受到抑制。在从高蜕皮激素时期获得的培养器官中观察到类似的选择性转录。我们进一步表明，在放线菌素 D 存在的情况下，Blimp-1 转录物很快消失。从这些结果，我们得出结论，当蜕皮激素效价较高时，Blimp-1 基因会转录，但表达的 mRNA 会迅速降解；这些独特的调节将其表达限制在高蜕皮类固醇阶段。
Blimp-1 is an ecdysone-inducible transcription factor that is expressed in the early stage of the prepupal period. The timing of its disappearance determines expression timing of the FTZ-F1 gene, whose temporally restricted expression is essential for the prepupal development. To elucidate the termination mechanism of Blimp-1 gene expression, we examined the regulation of the Blimp-1 gene using an organ culture system. The results showed that the Blimp-1 gene is transcribed in cultured organs taken from a low ecdysteroid period even after extended exposure to 20-hydroxyecdysone, while well-known early genes such as E75A are repressed under the same conditions. Similar selective transcription was observed in the cultured organs obtained from a high ecdysteroid period. We further showed that Blimp-1 transcripts quickly disappeared in the presence of actinomycin D. From these results, we concluded that the Blimp-1 gene is transcribed when the ecdysteroid titer is high, but the expressed mRNA degrades rapidly; these unique regulations limit its expression to the high ecdysteroid stage.