Quantitative assessment of antibodies to ribonucleoproteins in primary Sjogren syndrome: correlation with B-cell biomarkers and disease activity
Quantitative assessment of antibodies to ribonucleoproteins in primary Sjogren syndrome: correlation with B-cell biomarkers and disease activity
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DOI：
10.1136/ard.2008.095257
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发表时间：
2009-07-01
影响因子：
27.4
通讯作者：
Mariette, X.
中科院分区：
文献类型：
--
作者：
Candon, S.;Gottenberg, J. E.;Mariette, X.
目的：比较放射配基分析(RLA)和酶联免疫吸附试验(ELISA)检测SSA、SSB和RNP抗体的附加值，并分析自身抗体水平、B细胞标志物与疾病活动性的相关性。同时测定血清肿瘤坏死因子家族B细胞激活因子(BLyS，BAFF)水平。结果：RLA在检测SSB抗体(54%)和RNP抗体(9%)方面均优于EL ISA法(分别为5 4%和37%)。SSA抗体检测的敏感性差异无统计学意义。抗-SSA和抗SSB水平与两种技术均呈正相关。同时提供SSA和SSB抗体的患者的平均SSA抗体水平显著高于仅表现SSA抗体的患者(RLA：平均抗SSA水平分别为2343(158cpm)比1348(286cpm，p=0.02；ELISA：6.8(0.8cpm)vs3.8(0.4)，p=0.003)。SSA和sSb抗体水平与循环血中sSb和rnP水平显著相关(分别为r=0.4，p=0.004和r=0.6，p&t；0.001)，并与B细胞标志物(包括丙种球蛋白、β(2)微球蛋白和类风湿因子)水平显著相关。自身抗体的定量评估可能会揭示疾病活动性的生物标志物，并使人们能够进一步深入了解自身抗体反应扩散的机制。
Objectives: To assess the added value of using a radioligand assay (RLA) compared with ELISA to detect antibodies to SSA, SSB and RNP, and to analyse the correlation between autoantibody levels, B-cell biomarkers and disease activity.Patients and methods: Antibodies to SSA, SSB and RNP were assessed in 127 patients with primary Sjogren syndrome (pSS) using an RLA and ELISA. In parallel, measures of B-cell activation were determined including serum levels of B-cell-activating factor of the tumour necrosis factor family or BLyS (BAFF).Results: RLA was more sensitive than ELISA for the detection of antibodies to SSB (54% of positive samples versus 37%, respectively) and antibodies to RNP (9% vs 3%). No difference was seen for the sensitivity of detection of antibodies to SSA. Anti-SSA and anti-SSB levels were correlated with both techniques. Mean levels of antibodies to SSA were significantly higher in patients presenting antibodies to both SSA and SSB than in those exhibiting antibodies to SSA only (RLA: mean (SEM) anti-SSA levels 2343 (158) cpm vs 1348 (286) cpm, respectively, p = 0.02; ELISA: 6.8 (0.8) vs 3.8 (0.4), respectively, p = 0.003). Levels of antibodies to SSA and SSB significantly correlated with those of circulating BAFF (r = 0.4, p = 0.004 and r = 0.6, p < 0.001, respectively) and with B-cell biomarkers, including levels of gamma-globulins, beta(2) microglobulin and rheumatoid factor.Conclusion: RLA allowed a quantitative and more sensitive detection of antibodies to SSB and RNP in pSS. Quantitative assessment of autoantibodies might disclose a biomarker of disease activity and enable further insight into the pathogenesis of the spreading of the autoantibody response.