Polyadenylylated RNA complementary to a mouse retrovirus-like multigene family is rapidly and specifically induced by epidermal growth factor stimulation of quiescent cells.
Polyadenylylated RNA complementary to a mouse retrovirus-like multigene family is rapidly and specifically induced by epidermal growth factor stimulation of quiescent cells.
复制标题
与小鼠逆转录病毒样多基因家族互补的多聚腺苷酸化RNA可通过表皮生长因子刺激静止细胞而快速且特异性地诱导。
DOI：
10.1073/pnas.79.23.7317
复制
发表时间：
1982
影响因子：
11.1
通讯作者：
Getz,MJ
中科院分区：
文献类型：
--
作者：
Foster,DN;Schmidt,LJ;Hodgson,CP;Moses,HL;Getz,MJ
从总的多聚核糖体poly（A）+RNA群体制备的互补DNA探针用于鉴定小鼠DNA克隆，所述小鼠DNA克隆含有在表皮生长因子（EGF）刺激培养的静止小鼠胚胎细胞后特异性增强表达的序列。分离出三个这样的克隆，并用于研究EGF促有丝分裂刺激后细胞多核糖体中克隆特异性poly（A）+RNA水平的变化。刺激后6小时，与这些克隆中存在的序列互补的RNA占总poly（A）+RNA的比例增加了约10倍。通过杂交标准发现所有三个克隆都含有与称为VL30的小鼠逆转录病毒或转座子样元件类相关的序列。进一步发现这些VL30相关序列与EGF诱导的poly（A）+RNA互补，并且早在EGF刺激后1小时就可检测到增强的表达。相比之下，9个额外的克隆，包括AKR型小鼠白血病前病毒DNA克隆，不含可检测的VL30序列元件，并与poly（A）+RNA种类互补，其相对浓度在静止和EGF刺激的细胞中基本恒定。因此，VL30序列元件的不同之处在于，它们包括其表达响应于确定的肽生长因子而被特异性调节的成员。
Complementary DNA probes prepared from total polysomal poly(A)+RNA populations were used to identify clones of mouse DNA containing sequences whose expression is specifically enhanced after epidermal growth factor (EGF) stimulation of quiescent mouse embryo cells in culture. Three such clones were isolated and used to study changes in the levels of clone-specific poly(A)+RNA in the polysomes of cells after mitogenic stimulation by EGF. RNA complementary to sequences present in these clones increased approximately equal to 10-fold as a fraction of the total poly(A)+RNA by 6 hr after stimulation. All three clones were found by hybridization criteria to contain sequences related to the class of mouse retrovirus or transposon-like elements termed VL30. These VL30-related sequences were further found to be complementary to EGF-inducible poly(A)+RNAs and enhanced expression was detectable as early as 1 hr after EGF stimulation. In contrast, nine additional clones, including an AKR-type murine leukemia provirus DNA clone, contained no detectable VL30 sequence elements and were complementary to poly(A)+RNA species whose relative concentration was essentially constant in quiescent and EGF-stimulated cells. Therefore, VL30 sequence elements appear distinct in that they encompass members whose expression is specifically regulated in response to a defined peptide growth factor.