The stage of leaf maturity implicates the response of the photosynthetic apparatus to cadmium toxicity
The stage of leaf maturity implicates the response of the photosynthetic apparatus to cadmium toxicity
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DOI：
10.1016/s0168-9452(98)00159-9
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发表时间：
1998-11-23
期刊：
影响因子：
5.2
通讯作者：
Moniak, M
中科院分区：
文献类型：
--
作者：
Krupa, Z;Moniak, M
选择暴露于营养培养基中有毒浓度镉的黑麦幼苗的第一片叶子来研究叶子成熟阶段与光合装置对这种重金属的响应之间的关系。由于单子叶植物叶子的生长特征，叶子成熟的所有阶段都沿着这个器官呈现——从底部最年轻的细胞到叶尖最古老的细胞。最近对双子叶植物进行的一些研究证明，重金属毒性水平与叶龄及其生长阶段有关。我们决定在单子叶植物上测试这一假设，人们可以观察一片叶子的不同部分的所有成熟阶段。进行了基本生长分析（总叶长、叶切片的 FW 含量）、单个叶切片中 Cd 含量和叶绿体色素水平的测定、使用叶绿素荧光技术测量光合装置效率。我们已经表明，黑麦植物的叶片成熟阶段与光合装置的效率之间存在密切关系，其中卡尔文循环对镉毒性最敏感。 (C) 1998 Elsevier Science Ireland Ltd. 保留所有权利。
The first leaves of rye seedlings exposed to toxic concentration of Cd in the nutrient medium were chosen to study the relationship between the stage of leaf maturity and the response of the photosynthetic apparatus to this heavy metal. Because of growth characteristics of leaves of monocotyledonous plants all stages of leaf maturity are represented along this organ - from the youngest cells at the base to the oldest at the tip of the leaf. Some recent studies carried on dicotyledonous plants proved the level of heavy metals toxicity to be related to the leaf age and its stage of growth. We decided to test this hypothesis on monocotyledonous plants where one can observe all stages of maturity within different sections of one leaf. Basic growth analysis (total leaf length, FW content of leaf sections), determination of Cd content and chloroplast pigment levels in individual leaf sections, measurements of the photosynthetic apparatus efficiency using chlorophyll fluorescence techniques, were carried out. We have shown a close relation between the stage of leaf maturity and the efficiency of the photosynthetic apparatus in rye plants with the Calvin cycle being the most sensitive to Cd toxicity. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.