The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments
The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments
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DOI：
10.1373/clinchem.2013.206375
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发表时间：
2013-06-01
影响因子：
9.3
通讯作者：
Bustin, Stephen A.
中科院分区：
文献类型：
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作者：
Huggett, Jim F.;Foy, Carole A.;Bustin, Stephen A.
人们对数字PCR（dPCR）的兴趣越来越大，因为技术进步使其成为一种实用且越来越实惠的技术。dPCR允许核酸的精确定量，便于测量小百分比差异和定量罕见变体。与定量实时PCR（qPCR）相比，dPCR也可能更具重现性，并且对抑制更不敏感。因此，dPCR有可能对研究和诊断应用产生重大影响。然而，与qPCR一样，进行稳健的有意义实验的能力需要仔细的设计和充分的控制。为协助独立评估实验数据，需要全面披露所有相关实验细节。为了促进这一过程，我们提出了出版定量数字PCR实验指南的最低信息。本报告讨论了在其开发和商业实施的早期阶段已经确定的dPCR的已知要求。科学界采用这些准则将有助于标准化实验方案，最大限度地有效利用资源，并增强这一有前途的新技术的影响。(c)2013年美国临床化学协会
There is growing interest in digital PCR (dPCR) because technological progress makes it a practical and increasingly affordable technology. dPCR allows the precise quantification of nucleic acids, facilitating the measurement of small percentage differences and quantification of rare variants. dPCR may also be more reproducible and less susceptible to inhibition than quantitative real-time PCR (qPCR). Consequently, dPCR has the potential to have a substantial impact on research as well as diagnostic applications. However, as with qPCR, the ability to perform robust meaningful experiments requires careful design and adequate controls. To assist independent evaluation of experimental data, comprehensive disclosure of all relevant experimental details is required. To facilitate this process we present the Minimum Information for Publication of Quantitative Digital PCR Experiments guidelines. This report addresses known requirements for dPCR that have already been identified during this early stage of its development and commercial implementation. Adoption of these guidelines by the scientific community will help to standardize experimental protocols, maximize efficient utilization of resources, and enhance the impact of this promising new technology. (c) 2013 American Association for Clinical Chemistry