Long non-coding RNA AK055347 is upregulated in patients with atrial fibrillation and regulates mitochondrial energy production in myocardiocytes
Long non-coding RNA AK055347 is upregulated in patients with atrial fibrillation and regulates mitochondrial energy production in myocardiocytes
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DOI：
10.3892/mmr.2016.5893
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发表时间：
2016-12-01
影响因子：
3.4
通讯作者：
Liu, Chang
中科院分区：
文献类型：
--
作者：
Chen, Guiying;Guo, Hong;Liu, Chang
长链非编码RNA（lncRNA）在房颤中的作用仍有待充分阐明。当前的研究进行了微阵列分析，以研究16名房颤（AF）患者肺静脉和周围左心房区（LA-PV）以及左心耳（LAA）心房样本中lncRNA的差异表达谱。微阵列分析鉴定了94个在AF患者LA-PV和LAA之间差异表达的lncRNA。AK 055347是观察到的最显著改变的lncRNA之一。AK 055347的敲低抑制H9 C2心肌细胞的细胞活力，伴随Cyp 450和ATP酶的下调。此外，微阵列分析鉴定了MSS 51是AK 055347的靶标。AK 055347的敲低抑制H9 C2细胞中MSS 51的表达。本研究的观察结果表明，lncRNA在AF患者的LA-PV和LAA中差异表达。AK 055347可能通过调节Cyp 450、ATP合酶和MSS 51而使线粒体能量产生失调，从而导致AF的发病机制。
The role of long non-coding RNAs (lncRNAs) in atrial fibrillation remains to be fully elucidated. The current study performed microarray analysis to investigate differential lncRNA expression profiling in atrial samples from the pulmonary vein and the surrounding left atrial area (LA-PV) and from the left atrial appendage (LAA) in 16 patients with atrial fibrillation (AF). Microarray analysis identified 94 lncRNAs that were differentially expressed between the LA-PV and LAA in patients with AF. AK055347 was one of lncRNAs with the most significant alterations observed. Knockdown of AK055347 inhibited cell viability of H9C2 cardiomyocytes, accompanied by downregulation of Cyp450 and ATP synthases. In addition, microarray analysis identified that MSS51 was a target of AK055347. Knockdown of AK055347 inhibited the expression of MSS51 in H9C2 cells. The observations of the current study suggest that lncRNAs are differentially expressed in the LA-PV and LAA in patients with AF. AK055347 may contribute to the pathogenesis of AF by dysregulating mitochondrial energy production via regulation of Cyp450, ATP synthase and MSS51.