Berry anthocyanins:: isolation, identification and antioxidant activities
Berry anthocyanins:: isolation, identification and antioxidant activities
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DOI：
10.1002/jsfa.1511
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发表时间：
2003-11-01
影响因子：
4.1
通讯作者：
Heinonen, M
中科院分区：
文献类型：
--
作者：
Kähkönen, MP;Heinämäki, J;Heinonen, M
从越橘、黑加仑和越橘中分离出花青素用于抗氧化评估。使用 HPLC 和 HPLC/ESI-MS 技术对各个化合物进行鉴定和定量。在体外乳化亚油酸甲酯和人低密度脂蛋白 (LDL) 以及 2,2-二苯基-1-三硝基苯肼 (DPPH) 测试中研究了分离株的抗氧化和自由基清除能力。越橘、黑加仑和越橘酚类提取物中总花色苷含量分别为鲜重6000、2360和680mgkg(-1)。黑加仑中含有 4 种主要化合物（花青素和飞燕草苷的糖苷和芸香甙），越橘中含有 3 种主要化合物（花青素单苷），越橘中含有 15 种主要化合物（花青素、飞燕草苷、锦葵素、芍药苷和矮牵牛苷）。与基于相应标准化合物的定量相比，花青素-3-葡萄糖苷当量的定量在总花青素浓度以及单个飞燕草素和锦葵素化合物的含量方面给出的结果明显较低。浆果花青素在 DPPH 测试中是高活性的自由基清除剂，在乳液和人类 LDL 中是有效的抗氧化剂。 (C) 2003 年化学工业学会。
Anthocyanins from bilberry, blackcurrant and cowberry were isolated for antioxidant evaluation. Individual compounds were identified and quantified using HPLC and HPLC/ESI-MS techniques. Antioxidant and radical-scavenging capacities of the isolates were studied in emulsified methyl linoleate and human low-density lipoprotein (LDL) in vitro and in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test. The total anthocyanin contents in the phenolic extracts of bilberry, blackcurrant and cowberry were 6000, 2360 and 680 mgkg(-1) fresh weight respectively. There were four dominant compounds in blackcurrant (glucosides and rutinosides of cyanidin and delphinidin), three in cowberry (monoglycosides of cyanidin) and 15 in bilberry (monoglycosides of cyanidin, delphinidin, malvidin, peonidin and petunidin). Quantification as cyanidin-3-glucoside equivalents gave markedly lower results regarding the total anthocyanin concentration and the content of individual delphinidin and malvidin compounds compared with quantification based on corresponding standard compounds. Berry anthocyanins were highly active radical scavengers in the DPPH test and effective antioxidants in emulsion and human LDL. (C) 2003 Society of Chemical Industry.